关键词: 全自动固相萃取, 气相色谱质谱法, β-受体激动剂, 熟肉制品

Abstract: Objective To purify the samples with automatic-solid phase extraction instrument,and develop a method for determination of four β-receptor agonist residues in cooked meat products by gas chromatography mass spectrometry. Methods The samples were firstly digested with β-glucosidase/arylsulfatase overnight at 37 ℃,purified with automatic solid phase extraction instrument,derived with Bis（trimethylsilyl） trifluoroacetamide+1% trimethylchlorosilane,separated with TG-5MS capillary gas chromatography column,finally determined by gas chromatography mass spectrometry after separation by temperature programmed,and quantified by internal standard method. Results The method has good separation effect under the conditions,the correlation coefficients of the four target compounds were >0.999,the recoveries were 80.12%-90.16%,and the relative standard deviation（RSD） was 1.98%-6.20%（n=6）. Conclusion The method is fast and simple for pretreatment,with high sensitivity and good reproducibility,and is relatively friendly to operators and the environment,which is suitable for the determination of four β-receptor agonist residues in cooked meat products.

Key words: Automatic-solid phase extraction, Gas chromatography mass spectrometry, β-receptor agonist, Cooked meat products