Abstract: Objective To develop a method for determination of 12 polycyclic aromatic hydrocarbons metabolites in urine by ultra-performance liquid chromatography tandem mass spectrometry. Methods In 2022,the healthy adults from Shenzhen City were selected to collect their urine samples,the samples were hydrolyzesd by β-glucuronidase,purified and enriched by C₁₈ solid phase extraction cartridges,concentrated by nitrogen blowing,separated by Waters Acquity UPLC HSS T3 C₁₈ column（100 mm×2.1 mm×1.8 μm） and finally determined with UPLC-MS/MS under select response monitoring mode,quantified by internal standard method. Results 12 polycyclic aromatic hydrocarbons metabolites have a good linear relationship in range of 0.01-200 ng/mL,the correlation coefficients were above 0.997 0. The limits of detection were 0.002-0.041 ng/mL,the lower limits of quantitation were 0.007-0.135 ng/mL,and the sample spiking recovery rates were 88.4%-119.2%.The relative standard deviations（RSD） of intra batch precision were 2.8%-10.3%,and the RSD of inter batch precision were 3.1%-10.7%. Conclusion This method has the advantages of easy operation,high sensitivity,high precision,low detection limit,and it issuitable for the determination of 12 polycyclic aromatic hydrocarbons metabolites in urine.

Key words: Polycyclic aromatic hydrocarbons metabolites, Urine, Solid phase extraction, Ultra-performance liquid chromatography tandem mass spectrometry