Simultaneous determination of 20 alkaloids in cosmetics by QuEChERS-high performance liquid chromatography-tandem mass spectrometry

Abstract

Abstract: Objective By optimizing extraction conditions,purification conditions and instrument conditions,to establish a high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS） method for the simultaneous determination of 20 alkaloids in three cosmetic substrates. Methods The sample was extracted by ultrasonic with acetonitrile-water（7 ∶ 3,V/V） mixed solvent containing 1% formic acid,purified by QuEChERS technology,separated by Waters XBrige^TM C₁₈ liquid chromatography column,detected by multiple reaction monitoring（MRM） in positive mode of electric spray ion source（ESI）,and quantified by external standard method. Results The 20 alkaloids had a good linear relationship in the three cosmetics substrates（r>0.996）. The limits of detection and quantitation were 0.015-1.500 μg/kg and 0.049-4.900 μg/kg respectively. The average recoveries of 20 alkaloids were 64.0%-122.0%,and the relative standard deviations were 0.8%-13.3%. Conclusion The method is simple and easy to operate,with high sensitivity,good repeatability and accurate quantification,and is suitable for the detection and safety evaluation of various alkaloids in cosmetics.

Key words: Cosmetics, Alkaloids, High performance liquid chromatography-tandem mass spectrometry, QuEChERS, Ultrasonic extraction

CLC Number:

R115

ZHU Meng, LAI Miaoju, XU Siqi, XIONG Yanlan, CUI Shudan, LI Qiang. Simultaneous determination of 20 alkaloids in cosmetics by QuEChERS-high performance liquid chromatography-tandem mass spectrometry. [J]OCCUPATION AND HEALTH, 2024, 40(17): 2346-2351.

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