Abstract: Objective To understand the levels of vitamin A,vitamin D,and serum zinc among primary and middle school students in Pudong New Area of Shanghai City,and explore their impact on students' physical growth and development. Methods According to the requirements of the 'Nutrition Improvement Program for Compulsory Education Students in Rural Areas'（shorten as Student Nutrition Improvement Program）,a stratified random cluster sampling was used to select 605 primary and middle school students on a class by class basis. In October 2021,the students' height and body mass were measured,and fasting venous blood was collected to detect vitamin A,vitamin D,and serum zinc concentrations. Results The average age of the survey subjects was （10.53±2.64）years old,and the average concentration of vitamin A was （0.39±0.07）μg/mL,the average concentration of vitamin D was （17.97±5.82）ng/mL,and the average concentration of serum zinc was （152.71±85.96）μg/dL. The marginal deficiency rate of vitamin A was 10.58%,the deficiency rate of vitamin D was 52.89%,the marginal deficiency rate of vitamin D was 13.88%,and the deficiency rate of serum zinc was 15.70%. The rates of vitamin D deficiency and marginal deficiency in middle school students were higher than those in primary school students,and the rate was higher among girls than boys. The serum zinc deficiency rate in suburban areas was much higher than that in urban areas,and the differences were statistically significant（all P<0.01）. The marginal deficiency rate of vitamin A in lean individuals was higher,while the marginal deficiency rate of vitamin A in obese individuals was lower,with a statistically significant difference（P<0.01）. Conclusion Primary and secondary school students in Pudong New Area of Shanghai City,all suffer from deficiencies in vitamin A,vitamin D,and serum zinc,with vitamin D deficiency being particularly prominent and requiring high attention. Thin individuals are more prone to vitamin A deficiency.

Key words: Vitamin A, Vitamin D, Serum zinc, Lack, Primary and secondary school students, Physical growth