Determination of oxybenzone in drinking water by SPE-HPLC-MS

Abstract

Abstract: Objective To establish a solid phase extraction-high performance liquid chromatography-tandem mass spectrometry （SPE-HPLC-MS/MS） method for the determination of oxybenzone residues in domestic drinking water,and to detect the residual oxybenzoneinactual domestic drinking water. Methods HPLC-MS/MS method was established by using SPE for sample pretreatment,a C₁₈ column,methanol and ultrapure water as the mobile phases,a binary high-pressure gradient elution,multi-reaction monitoringmode for monitoring,and electrospraying+mode for determining. Results The linear range of oxybenzone was 0.200-500 μg/L with the regression equation of y=26 578x-4882,r=0.998 8,the relative standard deviation was 3.44%,and the limits of detection of the method were 0.005 0 ng/L,the limits of quantification were 0.017 ng/L,and the recoveries of the spiked standards ranged from 79.9 to 101.0%. Oxybenzone residues were detected in water samples taken in Chengdu,with average concentration of 6.43 ng/L. Conclusion The established method is sensitive,rapid,and can meet the requirements for the detection of oxybenzone residue in drinking water,and can provide an experimental basis for the subsequent study of oxybenzone residue detection.

Key words: Oxybenzone, Drinking water, Solid phase extraction, High performance liquid chromatography-tandem mass spectrometry

CLC Number:

R115

ZHANG Wenjiao, HE Yuandong, LI Ming, YAO Yuqin, SHI Ying. Determination of oxybenzone in drinking water by SPE-HPLC-MS. [J]OCCUPATION AND HEALTH, 2025, 41(10): 1351-1355.

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