Determination of 20 metal elements in blood by microwave digestion-inductively coupled plasma mass spectrometry

Abstract

Abstract:

Objective To establish an inductively coupled plasma mass spectrometry（ICP-MS） method for the simultaneous determination of 20 metal elements in blood，and to detect the metal exposure level of occupational population in the lithium battery dismantling industry. Methods Microwave digestion was employed as sample pretreatment，with digestion in a nitric acid system，acid scavenging，and dilution to a constant volume. The concentrations of lithium（Li），manganese（Mn），cobalt（Co），nickel（Ni），silver（Ag），arsenic（As），cadmium（Cd），chromium（Cr），lead（Pb），tin（Sn），strontium（Sr），antimony（Sb），vanadium（V），barium（Ba），titanium（Ti），beryllium（Be），molybdenum（Mo），thallium（Tl），aluminum（Al），and copper（Cu） in blood were determined under the standard mode（STD） and kinetic discrimination mode（KED） of ICP-MS. The matrix effect was reduced by internal standard calibration. Results The correlation coefficients（R²） of the linear equations for all 20 metal elements were all greater than 0.996. The detection limits ranged from 0.005 to 0.429 μg/L，and the quantification limits ranged from 0.015 to 1.431 μg/L. The recovery ranged from 85.36% to 109.14%，and the relative standard deviation（RSD） was ≤12.17%. Conclusion The established method is rapid，with good precision and accuracy，and suitable for the simultaneous determination of multiple metal elements in blood.

Key words: Microwave digestion, Inductively coupled plasma mass spectrometry, Blood, Metal elements

CLC Number:

R115

HOU Xintong, YANG Lixin, LI Yandong, LI Zengmin, ZHENG Hui, SHI Hongmei. Determination of 20 metal elements in blood by microwave digestion-inductively coupled plasma mass spectrometry. [J]OCCUPATION AND HEALTH, 2026, 42(18): 2496-2501.

Figures/Tables 7

References 29

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参数	设定值	参数	设定值
等离子体功率	1550 W	冷却气流量	14 L/min
进样方式	蠕动泵	扫描次数	3次
蠕动泵转速	40 r/min	样品提取时间	30 s
喷雾器流量	1.015 mL/min	采样深度	5 mm

参数	设定值	参数	设定值
等离子体功率	1550 W	冷却气流量	14 L/min
进样方式	蠕动泵	扫描次数	3次
蠕动泵转速	40 r/min	样品提取时间	30 s
喷雾器流量	1.015 mL/min	采样深度	5 mm

元素	线性范围（μg/L）	分析模式的选择	内标元素的选择	相关系数	检出限（μg/L）	定量限（μg/L）
⁷Li	0~100	STD	Y	0.999	0.016	0.055
⁹Be	0~100	STD	Y	0.999	0.014	0.045
²⁷Al	0~400	STD	Y	0.996	0.429	1.431
⁴⁸Ti	0~100	STD	Y	0.999	0.035	0.116
⁵¹V	0~100	KED	Rh	0.999	0.097	0.326
⁵²Cr	0~100	KED	Rh	0.997	0.106	0.353
⁵⁵Mn	0~100	KED	Rh	0.999	0.046	0.152
⁵⁹Co	0~100	KED	Rh	0.999	0.007	0.025
⁶⁰Ni	0~100	KED	In	0.999	0.239	0.795
⁶³Cu	0~2^a	STD	Y	0.998	0.158	0.528
⁷⁵As	0~100	KED	In	0.997	0.099	0.331
⁸⁸Sr	0~100	STD	Y	0.999	0.015	0.049
⁹⁵Mo	0~100	STD	Y	0.998	0.170	0.565
¹⁰⁷Ag	0~100	STD	Ge	0.999	0.049	0.164
¹¹¹Cd	0~100	STD	Ge	0.998	0.107	0.356
¹¹⁸Sn	0~100	KED	Rh	0.997	0.032	0.106
¹²¹Sb	0~100	STD	Ge	0.998	0.074	0.246
¹³⁷Ba	0~100	STD	Y	0.999	0.104	0.346
²⁰⁵Tl	0~100	STD	Ge	0.999	0.005	0.015
²⁰⁸Pb	0~100	STD	Ge	0.999	0.012	0.042

元素	线性范围（μg/L）	分析模式的选择	内标元素的选择	相关系数	检出限（μg/L）	定量限（μg/L）
⁷Li	0~100	STD	Y	0.999	0.016	0.055
⁹Be	0~100	STD	Y	0.999	0.014	0.045
²⁷Al	0~400	STD	Y	0.996	0.429	1.431
⁴⁸Ti	0~100	STD	Y	0.999	0.035	0.116
⁵¹V	0~100	KED	Rh	0.999	0.097	0.326
⁵²Cr	0~100	KED	Rh	0.997	0.106	0.353
⁵⁵Mn	0~100	KED	Rh	0.999	0.046	0.152
⁵⁹Co	0~100	KED	Rh	0.999	0.007	0.025
⁶⁰Ni	0~100	KED	In	0.999	0.239	0.795
⁶³Cu	0~2^a	STD	Y	0.998	0.158	0.528
⁷⁵As	0~100	KED	In	0.997	0.099	0.331
⁸⁸Sr	0~100	STD	Y	0.999	0.015	0.049
⁹⁵Mo	0~100	STD	Y	0.998	0.170	0.565
¹⁰⁷Ag	0~100	STD	Ge	0.999	0.049	0.164
¹¹¹Cd	0~100	STD	Ge	0.998	0.107	0.356
¹¹⁸Sn	0~100	KED	Rh	0.997	0.032	0.106
¹²¹Sb	0~100	STD	Ge	0.998	0.074	0.246
¹³⁷Ba	0~100	STD	Y	0.999	0.104	0.346
²⁰⁵Tl	0~100	STD	Ge	0.999	0.005	0.015
²⁰⁸Pb	0~100	STD	Ge	0.999	0.012	0.042

元素	低浓度			中浓度			高浓度
元素	加标量（μg/L）	回收率（%）	精密度（%）	加标量（μg/L）	回收率（%）	精密度（%）	加标量（μg/L）	回收率（%）	精密度（%）
⁷Li	4.00	98.50	7.77	20.00	94.15	5.61	100.00	88.60	6.90
⁹Be	4.00	94.50	7.64	20.00	91.70	6.39	100.00	91.20	9.53
²⁷Al	20.00	94.43	12.17	100.00	100.35	10.83	400.00	105.23	8.96
⁴⁸Ti	4.00	91.53	3.10	20.00	89.59	4.10	100.00	86.54	3.10
⁵¹V	4.00	101.00	3.00	20.00	102.26	7.16	100.00	95.20	7.80
⁵²Cr	4.00	100.00	5.49	20.00	102.03	5.19	100.00	99.54	7.02
⁵⁵Mn	4.00	108.08	7.46	20.00	104.62	1.92	100.00	106.91	2.35
⁵⁹Co	4.00	97.39	2.09	20.00	99.09	2.13	100.00	101.17	1.57
⁶⁰Ni	4.00	105.89	6.17	20.00	101.20	3.18	100.00	102.12	4.99
⁶³Cu	400.00	109.14	4.71	800.00	101.90	8.41	1200.00	96.51	7.97
⁷⁵As	4.00	99.50	7.24	20.00	97.33	6.64	100.00	96.12	6.40
⁸⁸Sr	4.00	98.38	3.42	20.00	96.99	6.67	100.00	99.77	5.68
⁹⁵Mo	4.00	101.41	6.46	20.00	96.39	5.42	100.00	97.37	3.86
¹⁰⁷Ag	4.00	88.92	5.29	20.00	91.34	6.28	100.00	91.36	3.65
¹¹¹Cd	4.00	97.92	6.38	20.00	96.04	7.72	100.00	91.80	5.58
¹¹⁸Sn	4.00	102.39	4.43	20.00	91.94	5.71	100.00	96.42	3.66
¹²¹Sb	4.00	99.33	5.19	20.00	90.06	6.51	100.00	91.34	5.04
¹³⁷Ba	4.00	93.00	9.37	20.00	94.23	8.07	100.00	101.08	4.10
²⁰⁵Tl	4.00	94.69	7.92	20.00	91.90	10.75	100.00	90.65	9.43
²⁰⁸Pb	4.00	89.03	6.09	20.00	101.30	7.47	100.00	91.55	4.77