基于SERS、侧流免疫分析及其联合技术对SARS-CoV-2检测现状及未来挑战

职业与健康 ›› 2023, Vol. 39 ›› Issue (10): 1421-1425.

基于SERS、侧流免疫分析及其联合技术对SARS-CoV-2检测现状及未来挑战

郑智恒¹, 张进¹, 刘艳坤², 王志武³, 李景武², 吴振刚¹

1.华北理工大学药学院,河北唐山 063210;
2.唐山市人民医院肿瘤防治研究所,河北唐山 063210;
3.唐山市人民医院放射化疗科,河北唐山 063210

收稿日期:2022-08-24 修回日期:2022-09-19 发布日期:2026-03-13
通信作者: 李景武,教授,E-mail：tslijingwu@163.com;吴振刚,教授,E-mail：327775309@qq.com
作者简介:郑智恒,男,在读硕士研究生,研究方向为病毒检测。
基金资助:
河北省自然科学基金项目（H2022209066）

Current status and future challenges of SARS-CoV-2 detection based on SERS,lateral flow immunoassay and its combined technology

ZHENG Zhi-heng¹, ZHANG Jin¹, LIU Yan-kun², WANG Zhi-wu³, LI Jing-wu², WU Zhen-gang¹

1. School of Pharmacy,North China University of Science and Technology,Tangshan Hebei 063210,China;
2. Institute of Cancer Prevention,Tangshan People's Hospital,Tangshan Hebei 063000,China;
3. Department of Radiotherapy and Chemotherapy,Tangshan People's Hospital,Tangshan Hebei 063000,China

Received:2022-08-24 Revised:2022-09-19 Published:2026-03-13
Contact: LI Jing-wu,Professor,E-mail：tslijingwu@163.com;WU Zhen-gang,Professor,E-mail：327775309@qq.com

摘要/Abstract

摘要： 严重急性呼吸综合症冠状病毒2（seveve acute respiratiory syndrome coronavivus 2,SARS-CoV-2）具有极高传染性,对人类生命安全造成极大威胁。SARS-CoV-2的早期和快速诊断在抗击疫情方面发挥着重要作用,需要一种快速、灵敏且廉价的检测系统。表面增强拉曼光谱（surface-enhanced raman scattering,SERS）作为一种前沿分析技术具备指纹识别能力,可提供具有高特异性、灵敏度和多重检测的定量结果,在病毒的即时诊断方面具有广阔前景,目前已被应用于多种甲型流感病毒亚型的检测。侧流免疫分析（lateral flow immunoassay,LFIA）等床旁（point of care,POC）设备提供了快速、高效、可靠的平台。然而,LFIA在SARS-CoV-2检测的定量和灵敏分析方面存在局限性。为了解决这些问题,本文综述了SERS技术与LFIA结合,用于SARS-CoV-2的定量分析现状及未来挑战。

关键词: 表面增强拉曼光谱, 严重急性呼吸综合症冠状病毒2, 侧流免疫分析, 未来挑战

Abstract: ：The severe acute respiratory syndrome coronavirus（SARS-CoV-2） is highly contagious,posing a great threat to human life and security. The early and rapid diagnosis of SARS-CoV-2 plays an important role in fighting outbreaks,and there is an urgent need for a rapid,sensitive and inexpensive test system. The surface-enhanced raman scattering（SERS）,as a cutting-edge analytical technique,has the ability of fingerprint recognition,which can provide quantitative results with high specificity,sensitivity and multiple detection,and has broad prospects in the immediate diagnosis of viruses.It has been applied in the detection of various influenza virus subtypes. The lateral flow immunoassay（LFIA） and other point of care（POC） devices provide a fast,efficient and reliable platform.However,LFIA has limitations in quantitative and sensitive analysis of SARS-CoV-2 detection.To solve these problems,we review the combination of SERS technology and LFIA in current status and future challenges of the quantitative analysis of SARS-CoV-2.

Key words: Surface-enhanced raman scattering, Severe acute respiratory syndrome coronavirus, Lateral flow immunoassay, Future challenges

中图分类号:

R373.1⁺9

郑智恒, 张进, 刘艳坤, 王志武, 李景武, 吴振刚. 基于SERS、侧流免疫分析及其联合技术对SARS-CoV-2检测现状及未来挑战. [J]职业与健康, 2023, 39(10): 1421-1425.

ZHENG Zhi-heng, ZHANG Jin, LIU Yan-kun, WANG Zhi-wu, LI Jing-wu, WU Zhen-gang. Current status and future challenges of SARS-CoV-2 detection based on SERS,lateral flow immunoassay and its combined technology. [J]OCCUPATION AND HEALTH, 2023, 39(10): 1421-1425.

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