自然吸入PM2.5致小鼠肾脏损伤相关炎症因子及FGF21在肾脏中的表达情况

职业与健康 ›› 2023, Vol. 39 ›› Issue (14): 1886-1890.

自然吸入PM_2.5致小鼠肾脏损伤相关炎症因子及FGF21在肾脏中的表达情况

王萌, 王莹, 张珉, 张忠, 李舒音, 王旭光

沈阳医学院病理教研室,辽宁沈阳 110034

收稿日期:2022-11-01 修回日期:2022-12-06 出版日期:2023-07-15 发布日期:2026-03-24
通信作者: 王旭光,教授,E-mail：wangxuguang@symc.edu.cn
作者简介:王萌,女,在读硕士研究生,研究方向为药物毒性病理肿瘤性病变的种属差异。
基金资助:
辽宁省科学技术厅项目（2019-ZD-0319）; 沈阳市环境污染与微生态重点实验室子项目（2018003）

Expression of inflammatory factors related to kidney injury and FGF21 in mice induced by natural inhalation of PM_2.5

WANG Meng, WANG Ying, ZHANG Min, ZHANG Zhong, LI Shuyin, WANG Xuguang

Department of Pathology,Shenyang Medical College,Shenyang Liaoning,110034,China

Received:2022-11-01 Revised:2022-12-06 Online:2023-07-15 Published:2026-03-24
Contact: WANG Xuguang,Professor,E-mail：wangxuguang@symc.edu.cn

摘要/Abstract

摘要： 目的探讨自然吸入细颗粒物（particulate matter 2.5,PM_2.5）致小鼠肾脏损伤相关炎症因子及成纤维细胞生长因子21（fibroblast growth factor 21,FGF21）在肾脏中的表达情况,推断在自然吸入PM_2.5致肾脏损伤时FGF21是否有望成为潜在的生物学指标。方法依据随机数字表法,将52只6周龄的健康雄性小鼠随机分为对照组（自然吸入空气）和实验组（PM_2.5染毒组）,每组26只。实验组自然吸入PM_2.5气溶胶28周。HE染色观察小鼠肾脏病理形态,免疫组织化学法检测肾脏中肿瘤坏死因子α（Tumor necrosis factor-α,TNF-α）、白细胞介素1β（Interleukin 1β,IL-1β）、白细胞介素6（Interleukin 6,IL-6）及FGF21蛋白表达情况,并对FGF21与TNF-α、IL-1β和IL-6的相关性进行分析。结果与对照组比较,实验组小鼠肾脏出现明显的病理损伤,差异有统计学意义（P<0.05）;与对照组比较,实验组小鼠肾脏TNF-α、IL-1β和IL-6蛋白表达水平升高,FGF21蛋白表达水平下降,差异均有统计学意义（均P<0.05）;FGF21与TNF-α、IL-1β和IL-6均存在负相关关系（均P<0.05）。结论 PM_2.5自然吸入可致小鼠肾脏发生明显损伤,抑制肾脏FGF21的表达,促进炎症因子TNF-α、IL-1β和IL-6的表达,FGF21与TNF-α、IL-1β和IL-6均存在负相关性。推测在自然吸入PM_2.5致肾脏损伤时FGF21有望成为潜在的生物学指标。

关键词: 细颗粒物, 肾脏损伤, 炎症因子, FGF21

Abstract: Objective To investigate the expression of inflammatory factors and fibroblast growth factor 21（FGF21） in the kidneys of mice induced by fine natural inhalation of particulate matter 2.5（PM_2.5）,inferwhether FGF21 is expected to be a potential biological indicator in kidney damage caused by natural inhalation of PM_2.5. Methods According to the random number table method,52 healthy male mice aged 6 weeks were randomly divided into the control group（natural inhalation of air） and the experimental group（PM_2.5 exposure group）,with 26 mice in each group. The experimental group naturally inhaled PM_2.5 aerosol for 28 weeks. HE staining was used to observe the pathological morphology of the mouse kidneys. The immunohistochemical method was used to detect the protein expressions of tumor necrosis factor-α（TNF-α）,interleukin 1β（IL-1β）,interleukin 6（IL-6）and fibroblast growth factor 21（FGF21）.And the correlation between FGF21 and TNF-α,IL-1β and IL-6 was analyzed.Results Compared with the control group,the kidneys of the mice in the experimental group had obvious pathological damage,and the difference was statistically significant（P<0.05）. Compared with the control group,the expression levels of TNF-α,IL-1β and IL-6 in the kidney of mice in the experimental group increased,while the expression level of FGF21 decreased,and the differences were statistically significant（all P<0.05）. The FGF21 was negatively correlated with TNF-α,IL-1β and IL-6（all P<0.05）. Conclusions The natural inhalation of PM_2.5 can cause significant kidney damage in mice,inhibit the expression of FGF21 in the kidney,and promote the inflammatory factor TNF-α,IL-1β and IL-6 expression. The FGF21 is negatively correlated with TNF-α,IL-1β and IL-6.It is speculated that FGF21 may become a potential biological indicator in kidney damage caused by natural inhalation of PM_2.5.

Key words: Fine particulate matter, Kidney injury, Inflammatory factor, FGF21

中图分类号:

R-332

王萌, 王莹, 张珉, 张忠, 李舒音, 王旭光. 自然吸入PM_2.5致小鼠肾脏损伤相关炎症因子及FGF21在肾脏中的表达情况. [J]职业与健康, 2023, 39(14): 1886-1890.

WANG Meng, WANG Ying, ZHANG Min, ZHANG Zhong, LI Shuyin, WANG Xuguang. Expression of inflammatory factors related to kidney injury and FGF21 in mice induced by natural inhalation of PM_2.5. [J]OCCUPATION AND HEALTH, 2023, 39(14): 1886-1890.

参考文献

[1] 孙静,张勉.PM_2.5对呼吸系统损伤的研究进展[J].中国当代医药,2020,14(6):26-29.
[2] 王金泉. 重视环境污染对肾脏的损害[J].肾脏病与透析肾移植杂志,2021,30(1):55-56.
[3] 索丹凤,曾三武.空气细颗粒物PM_2.5对人体各系统危害的研究[J].医学信息,2019,32(18):32-34.
[4] 赵剑刚. PM_2.5暴露与肾脏损害的研究进展[J].预防医学,2021,33(5):473-475.
[5] CHEN S Y,CHU D C,LEE J H,et al.Traffic-related air pollution associated with chronic kidney disease among elderly residents in Taipei City[J].Environ Pollut,2018,234(3):838-845.
[6] LUO B,WANG Z,ZHANG Z,et al.The deficiency of macrophage erythropoietin signaling contributes to delayed acute inflammation resolution in diet-induced obese mice[J].Biochim Biophys Acta Mol Basis Dis,2019,1865(2):339-349.
[7] 饶毅峰,杨林,杜京涛,等.糖尿病肾病患者尿白蛋白排泄率与肾功能、炎症反应、氧化应激反应的相关性[J].海南医学院学报,2017,23(6):769-772.
[8] ESTEGHAMATI A,KHANDAN A,MOMENI A,et al.Circulating levels of fibroblast growth factor 21 in early-stage diabetic kidney disease[J].Irish J Med Sci,2017,186(3).785-794.
[9] 黄乔木,何艳.尿视黄醇结合蛋白、尿肝型脂肪酸结合蛋白、血成纤维细胞生长因子-21、血转化生长因子-β联合诊断早期糖尿病肾病的价值[J].临床肾脏病杂志,2022,22(4):283-290.
[10] CRASTO C,SEMBA R D,SUN K,et al.Serum fibroblast growth factor 21 is associated with renal function and chronic kidney disease in community-dwelling adults[J].J Am Geriatr Soc,2012,60(4):792-793.
[11] 张俊峰,韩力慧,程水源,等.京津冀地区典型城市大气细颗粒物碳质组分污染特征及来源[J].环境科学研究,2020,33(8):1729-1739.
[12] 杜青,张予燕.ICP-MS法测定南京市环境空气 PM_2.5中多种元素[J].环境监测管理与技术,2017,29(2):45-49.
[13] 陈熙勐,张皓旻,顾万清,等.我国PM_2.5主要成分及对人体健康危害研究进展[J].中华保健医学杂志,2019,21(1):87-89.
[14] 史云洁,王沛,马姗婕,等.大气细颗粒物对实验动物氧化应激及炎症反应研究进展[J].中国公共卫生,2017,33(1):35-38.
[15] 黄强,董发勤,王利民,等.PM_2.5的细胞毒性及机制研究进展[J].毒理学杂志,2014,28(1):68-72.
[16] 于建军,师旭辉.IL-18、TNF-α、TGF-β1在IgA肾病中表达及其与病情进展的关系[J].辽宁医学杂志,2020,34(4):25-27.
[17] GE C X,QIN Y T,LOU D S,et al.Irhom2 deficiency relieves TNF-α associated hepatic dyslipidemia in long-term PM_2.5-exposed mice[J].Biochem Biophys Res Commun,2017,493(4):1402-1409.
[18] LIN C H,WAN C,LIU W S,et al.PM_2.5 Induces early epithelial mesenchymal transition in human proximal tubular epithelial cells through activation of IL-6/STAT3 Pathway[J].Int J Mol Sci,2021,22(23):12734.
[19] HE M,ICHINOSE T,YOSHIDA S,et al.PM_2.5-induced lung inflammation in mice:Differences of inflammatory response in macrophages and type II alveolar cells[J].J Appl Toxicol,2017,37(4):1203-1218.
[20] RASKING L,VANBRABANT K,BOVÉ H,et al.Adverse Effects of fine particulate matter on human kidney functioning:a systematic review[J].Environ Health,2022,21(1):24.
[21] 陈琰,高依依,李占园,等.慢性肾脏病患者及动物模型中成纤维细胞生长因子21的表达及意义[J].中国全科医学,2014,17(21):2462-2466.
[22] GAMROT Z,ADAMCZYK P,袁行行WI袁行行TOCHOWSKA E,et al.Fibroblast growth factor 21(FGF21) in children and adolescents with chronic kidney disease[J].Physiol Res,2020,69(3):451-460.
[23] 周晨曦,曹燕花,李清钊,等.PM_2.5致大鼠肾脏氧化损伤效应研究[J].环境与健康杂志,2019,36(7):571-574.
[24] 张简娜,卢国元,张骥,等.成纤维细胞生长因子FGF21与糖尿病肾病相关联的临床研究[J].浙江医学教育,2016,15(4):45-48.