2018—2021年北京市大兴区腹泻患者沙门菌分子分型及耐药性分析

职业与健康 ›› 2023, Vol. 39 ›› Issue (2): 170-174.

2018—2021年北京市大兴区腹泻患者沙门菌分子分型及耐药性分析

马琳, 温静, 冯光远, 张奇, 郑天驰, 王希峰

北京市大兴区疾病预防控制中心微生物检验科，北京 102600

收稿日期:2022-04-12 修回日期:2022-07-07 发布日期:2026-03-02
通信作者: 王希峰，主管检验师，E-mail：xifengwang@foxmail.com
作者简介:马琳，女，主管检验师，主要从事微生物检验工作。

Analysis on Molecular characteristics and antibiotic resistance of Salmonella spp isolated from diarrhea patients in Daxing District of Beijing from 2018-2021

MA Lin, WEN Jing, FENG Guang-yuan, ZHANG Qi, ZHENG Tian-chi, WANG Xi-feng

Microbiological Laboratory，Daxing District Center for Disease Control and Prevention，Beijing 102600，China

Received:2022-04-12 Revised:2022-07-07 Published:2026-03-02
Contact: WANG Xi-feng，Laboratorian in charge，E-mail: xifengwang@foxmail.com

摘要/Abstract

摘要： 目的了解北京市大兴区肠道门诊腹泻患者沙门菌的感染情况、流行病学和病原学特征及耐药状况，为沙门菌防控及临床治疗提供依据。方法 2018—2021年收集大兴区3家哨点医院肠道门诊腹泻患者的粪便标本进行沙门菌检测，对分离到的沙门菌开展血清分型、脉冲场凝胶电泳分型分析及药敏实验。结果 4年共检测粪便标本1 499件，检出沙门菌65株（4.34%），肠炎沙门菌（53.85%）为主要流行血清型;不同年龄组人群中沙门菌检出率差异有统计学意义（P<0.01），其中1~<11岁检出率最高（10.30%）;不同性别及职业人群沙门菌检出率差异均无统计学意义（均P>0.05）。药敏结果显示，沙门菌耐药程度差异较大，除对头孢西丁、美罗培南和阿奇霉素完全敏感外，对其他抗菌药物均有一定的耐药性。35株肠炎沙门菌脉冲场凝胶电泳后出现20种带型，其中B8和B10带型为优势带型，菌株之间的相似系数为40.6%~98.7%。结论北京市大兴区沙门菌的优势血清型为肠炎沙门菌，PFGE带型呈高度多态性，耐药性较强，临床治疗时需合理使用抗菌药物。

关键词: 沙门菌, 血清学鉴定, 耐药性, 分子分型, 脉冲场凝胶电泳

Abstract: Objective To understand the infection status，epidemiological and etiological characteristics，and drug resistance of Salmonella spp isolated from diarrhea patients in enteric diseases clinic of Daxing District in Beijing，provide evidence for the prevention，control and clinical treatment of Salmonella spp. Methods From 2018-2021，the stool samples of diarrhea patients in enteric diseases clinic of three sentinel hospitals in Daxing District were collected for testing Salmonella spp，and the isolated Salmonella spp were analyzed by serotyping，pulsed-field gel electrophoresis and antibiotics susceptibility test. Results A total of 1 499 stool samples were tested in four years，and 65 strains（4.34%） of Salmonella spp were detected. The main serotype was Salmonella enteritidis（53.85%）. The difference in the detection rate of Salmonella spp was statistically significant among different age groups（P<0.01），and the highest infection rate（10.30%） was in the age group of 1-<11 years old. There were statistically significant differences in the detection rate among groups in different age and occupation（all P>0.05）.The results of drug resistance test showed that there were great differences in the degree of drug resistance of Salmonella spp. Except that it was completely sensitive to cefoxitin，meropenem and azithromycin，it had certain drug resistance to other antibiotics. The pulsed field gel electrophoresisanalysis showed that 20 PFGE patterns were found in 35 Salmonella spp strains，with the dominant patterns of B8 and B10，and the similarity coefficients of strains were 40.6%-98.7%. Conclusion The Salmonella enteritidisis predominant serotype of Salmonella spp in Daxing District of Beijing，the PFGE patterns show polymorphism，and the drug resistance is strong，so the rational use of antibiotics should be strengthened in clinical treatment.

Key words: Salmonella spp, Serological identification, Drug resistance, Molecular genotype, Pulsed field gel electrophoresis

中图分类号:

R117

马琳, 温静, 冯光远, 张奇, 郑天驰, 王希峰. 2018—2021年北京市大兴区腹泻患者沙门菌分子分型及耐药性分析. [J]职业与健康, 2023, 39(2): 170-174.

MA Lin, WEN Jing, FENG Guang-yuan, ZHANG Qi, ZHENG Tian-chi, WANG Xi-feng. Analysis on Molecular characteristics and antibiotic resistance of Salmonella spp isolated from diarrhea patients in Daxing District of Beijing from 2018-2021. [J]OCCUPATION AND HEALTH, 2023, 39(2): 170-174.

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