2015—2020年深圳市水产品及养殖水中副溶血弧菌的毒力基因与耐药性分析

职业与健康 ›› 2023, Vol. 39 ›› Issue (21): 3011-3014.

2015—2020年深圳市水产品及养殖水中副溶血弧菌的毒力基因与耐药性分析

张金金^1a, 陈浩川^1b, 陈润莉^1a, 王家豪², 李玲玲^1a, 刘松^1a,3

1.深圳市福田区疾病预防控制中心 a微检科,b 传染病科,广东深圳 518040;
2.广东医科大学公共卫生学院,广东东莞 523808;
3.暨南大学附属第二临床医学院,深圳市人民医院（临床医学研究中心）,广东深圳 518020

收稿日期:2023-04-10 修回日期:2023-05-09 出版日期:2023-11-01 发布日期:2026-03-26
通信作者: 刘松,主管技师,E-mail：liusong9666@126.com
作者简介:张金金,女,副主任技师,主要从事微生物检测及病原菌分子分型工作。
基金资助:
深圳市福田区卫生公益性科研项目（FTWS2019073）; 深圳市福田区卫生公益性科研项目（FTWS2021079）; 深圳市福田区卫生公益性科研项目（FTWS2023084）; 国家自然科学基金（82003813）

Analysis of virulence genes and drug resistance of Vibrio parahaemolyticus in aquatic products and aquaculture water in Shenzhen City from 2015-2020

ZHANG Jinjin^1a, CHEN Haochuan^1b, CHEN Runli^1a, WANG Jiahao², LI Lingling^1a, LIU Song^1a,3

1. a Microbiological Laboratory,b Department of Infectious Diseases,Shenzhen Futian District Center for Disease Prevention and Control,Shenzhen Guangdong 518040,China;
2. School of Public Health,Guangdong Medical University,Dongguan Guangdong 523808,China;
3. Second Clinical Medical College Affiliated to Jinan University,Shenzhen People's Hospital（Clinical Medical Research Center）,Shenzhen Guangdong 518020,China

Received:2023-04-10 Revised:2023-05-09 Online:2023-11-01 Published:2026-03-26
Contact: LIU Song,Technician in charge,E-mail：liusong9666@126.com

摘要/Abstract

摘要： 目的了解2015—2020年深圳市水产品及养殖水中副溶血弧菌的毒力基因和耐药谱情况。方法采用多重荧光定量聚合酶链式反应（polymerase chain reaction,PCR）技术对来源于深圳市食品风险及霍乱监测中的96株副溶血弧菌进行耐热直接溶血素（thermostable direct hemolysin,TDH）、耐热直接相关溶血素（TDH-related hemolysin,TRH）和不耐热溶血素（thermolabile hemolysin,TLH）毒力基因筛查;采用最小抑菌浓度（minimum inhibitory concentration,MIC）法对其进行17种抗生素耐药谱筛查。结果 96株副溶血弧菌均不含有tdh和trh基因,tlh基因携带率为100%。菌株对氨苄西林耐药率最高,为93.75%,中介率为5.21%;对头孢唑啉中介率最高,为42.71%,其次是头孢吡肟和氨曲南,中介率分别为16.67%和10.42%;所有菌株对头孢替坦、头孢他啶、环丙沙星、庆大霉素、头孢曲松、呋喃妥因、亚胺培南、左旋氧氟沙星、妥布霉素9种抗生素均敏感,敏感率为100%。结论 2015—2020年深圳市水产品及养殖水中副溶血弧菌携带毒力情况较少,普遍对氨苄西林耐药,菌株对其他抗生素也存在一定程度耐药和中介,个别菌株出现多重耐药。

关键词: 副溶血弧菌, 耐药性, 水产品, 最小抑菌浓度, 抗生素

Abstract: Objective To understand the virulence genes and drug resistance spectrum of Vibrio parahaemolyticus in aquatic products and aquaculture water in Shenzhen City from 2015 to 2020. Methods The virulence genes of thermostable direct hemolysin（TDH）,TDH-related hemolysin（TRH） and thermolabile hemolysin（TLH） of 96 strains of Vibrio parahaemolyticus from the food risk and cholera surveillance in Shenzhen City were screened by multiplex fluorescence quantitative PCR technology. The resistance to 17 kinds of antibiotics was determined by the minimum inhibitory concentration（MIC）. Results None of the 96 strains of Vibrio parahaemolyticus contained tdh and trh genes,and the tlh gene carrier rate was 100%. The resistance rate of strains to ampicillin was the highest,which was 93.75%,and the intermediate rate was 5.21%. The intermediate rate to cefazolin was the highest,which was 42.71%,followed by cefepime and aztreonam,the intermediary rates were 16.67% and 10.42% respectively. All strains were sensitive to nine antibiotics including cefotetan,ceftazidime,ciprofloxacin,gentamicin,ceftriaxone,nitrofurantoin,imipenem,levofloxacin and tobramycin,and the sensitivity rate was 100%. Conclusion The virulence of Vibrio parahaemolyticus from aquatic products and aquaculture water in Shenzhen City from 2015 to 2020 is less,it is generally resistant to ampicillin,the strains also have a certain degree of resistance and intermediary to other antibiotics,and individual strains show multidrug resistance.

Key words: Vibrio parahaemolyticus, Drug resistance, Aquatic products, Minimum inhibitory concentration, Antibiotics

中图分类号:

R117

张金金, 陈浩川, 陈润莉, 王家豪, 李玲玲, 刘松. 2015—2020年深圳市水产品及养殖水中副溶血弧菌的毒力基因与耐药性分析. [J]职业与健康, 2023, 39(21): 3011-3014.

ZHANG Jinjin, CHEN Haochuan, CHEN Runli, WANG Jiahao, LI Lingling, LIU Song. Analysis of virulence genes and drug resistance of Vibrio parahaemolyticus in aquatic products and aquaculture water in Shenzhen City from 2015-2020. [J]OCCUPATION AND HEALTH, 2023, 39(21): 3011-3014.

参考文献

[1] 付萍,王连森,陈江,等.2015年中国大陆食源性疾病暴发事件监测资料分析[J].中国食品卫生杂志,2019,31(1):64-70.
[2] 秦郑,胡锦瑞,吴科明,等.我国东部沿海地区江河入海口水体中致病性弧菌分布特征[J].中国热带医学,2021,21(6):501-504.
[3] 马晓薇,李泳光,冯晶,等.2006—2019年广州市食物中毒突发公共卫生事件流行病学特征分析[J].实用预防医学,2021,28(9):1059-1062.
[4] 常方媛,赵久飞,董建凤,等.2014—2020年青岛市黄岛区食源性疾病暴发事件流行病学特征分析[J].预防医学论坛,2022,28(7):541-545.
[5] 张德福,安慧,张健,等.副溶血弧菌耐药及其机制的研究进展[J].食品工业科技,2018,39(9):311-317,324.
[6] 周倩,向婧姝,黄靖宇,等.贵阳市淡水产品及养殖环境致病性弧菌监测结果分析[J].应用预防医学,2022,28(4):352-354,396.
[7] 许金凤,曹文婷,张潇丹,等.镇江地区2018年不同来源副溶血性弧菌毒力基因及耐药性分析[J].临床检验杂志,2020,38(1):70-72.
[8] RYU A R,MOK J S,LEE D E,et al.Occurrence,virulence,and antimicrobial resistance of Vibrio parahaemolyticus isolated from bivalve shellfish farms along the southern coast of Korea[J].Environ Sci Pollut Res Int,2019,26(20):21034-21043.
[9] 中华人民共和国国家卫生和计划生育委员会.中华人民共和国国家标准:GB 4789.7—2013[S].北京:中国标准出版社,2014.
[10] 刘娜. 2016—2018年沈阳市海产品副溶血弧菌毒力基因与耐药性[J].环境与健康杂志,2019,36(10):936-937.
[11] 龙冬玲,杨春晓,董莉,等.珠海市市售水产品副溶血性弧菌病原学特征分析[J].医学动物防制,2021,37(12):1210-1214.
[12] 陈伟冰,李柏生,卢向明,等.广东省茂名市副溶血弧菌涉水产品分离株病原学特征[J].中国热带医学,2020,20(4):351-355.
[13] 阙凤霞,叶玉龙,杨博雯,等.上海市金山区2017年—2018年水产品中副溶血性弧菌病原学特征分析[J].中国卫生检验杂志,2020, 30(16):1964-1966.
[14] 庞梦婷,高巍,贠庆茹,等.2018—2019年包头市市售水产品中副溶血性弧菌监测结果及病原学特征分析[J].现代预防医学,2021, 48(22):4201-4205.
[15] 李丽倩,姚琳,林才云,等.2018年贝类来源副溶血性弧菌毒力基因及耐药性分析[J].中国食品卫生杂志,2020,32(4):364-370.
[16] 李平,黄涵,钟汶兵.海口市市售贝类海产品副溶血性弧菌污染调查及耐药和毒力基因研究[J].中国食品卫生杂志,2019,31(4):366-370.
[17] 郭辉,朱烁,韩雪,等.蚌埠市2018年水产品致病性弧菌污染情况检测结果分析[J].安徽预防医学杂志,2021,27(3):216-218.
[18] 马肖. 湖北两地区水产品中副溶血性弧菌多元性分析及研究[D].衡阳:南华大学,2017.
[19] LIU J,BAI L,LI W,et al.Trends of foodborne diseases in China:Lessons from laboratory-based surveillance since 2011[J].Front Med,2018,12(1):48-57.
[20] 蔡楠楠,吴莹,杜波,等.辽宁省阜新市副溶血性弧菌的生物学特征、毒力基因、耐药特征及分子溯源[J].中国热带医学,2021,21(8):724-729.
[21] 俞佳莉,乔雪飞,盛峰松,等.2016—2020年上海市松江区腹泻患者副溶血弧菌耐药性与分子分型研究[J].疾病监测,2022,37(2):245-250.
[22] 肖文静,陈美玲,赵文轩,等.tdh+与tdh-副溶血弧菌耐药表型与基因型差异分析[J].疾病监测,2021,36(5):489-494.
[23] ZAGO V,VESCHETTI L,PATUZZO C,et al.Shewanella algae and Vibrio spp.strains isolated in Italian aquaculture farms are reservoirs of antibiotic resistant genes that might constitute a risk for human health[J].Mar Pollut Bull,2020(154):111057.
[24] 韩昊男. 水产养殖中抗生素使用存在问题及改进措施[J].畜牧兽医科学(电子版),2020(15):149-150.
[25] 王高,王淼,柴云美,等.云南淡水鱼中副溶血性弧菌的污染情况及耐药性分析[J].食品安全质量检测学报,2020,11(9):2779-2784.