QuEChERS-高效液相色谱-串联质谱法同时测定化妆品中20种生物碱

职业与健康 ›› 2024, Vol. 40 ›› Issue (17): 2346-2351.

QuEChERS-高效液相色谱-串联质谱法同时测定化妆品中20种生物碱

朱猛, 赖淼菊, 徐斯琦, 熊艳蓝, 崔淑丹, 李强

上海市普陀区疾病预防控制中心理化检验科,上海 200333

收稿日期:2024-01-08 修回日期:2024-01-22 发布日期:2026-03-17
通信作者: 李强,副主任技师,E-mail：817125@163.com
作者简介:朱猛,男,主管技师,主要从事环境污染物检测工作。
基金资助:
上海市普陀区公共卫生青年课题（ptgw202107）; 上海市普陀区公共卫生重点学科-卫生理化检验学（ptgw202001）

Simultaneous determination of 20 alkaloids in cosmetics by QuEChERS-high performance liquid chromatography-tandem mass spectrometry

ZHU Meng, LAI Miaoju, XU Siqi, XIONG Yanlan, CUI Shudan, LI Qiang

Physical and Chemical Department,Shanghai Putuo District Center for Disease Control and Prevention,Shanghai 200333,China

Received:2024-01-08 Revised:2024-01-22 Published:2026-03-17
Contact: LI Qiang,Deputy chief technician,E-mail：817125@163.com

摘要/Abstract

摘要： 目的通过优化提取条件、净化条件、仪器条件等参数,建立一种同时测定3种化妆品基质中20种生物碱的高效液相色谱-串联质谱（high performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS）分析方法。方法样品经含1%甲酸的乙腈-水（7 ∶ 3,V/V）混合溶剂超声提取,QuEChERS技术净化,经Waters XBrige^TM C₁₈液相色谱柱分离后,在电喷雾离子源（electric spray ion source,ESI）正离子模式下通过多反应监测（multi-reaction monitoring,MRM）方式检测,外标法定量。结果 20种生物碱在3种化妆品基质中具有良好的线性关系（r>0.996）,方法的检出限为0.015~1.500 μg/kg,定量限为0.049~4.900 μg/kg,20种生物碱平均回收率为64.0%~122.0%,相对标准偏差为0.8%~13.3%。结论所建立的方法简便易操作、灵敏度高、重复性好、定量精准,适用于化妆品中多种生物碱的检测和安全性评价。

关键词: 化妆品, 生物碱, 高效液相色谱-串联质谱, QuEChERS, 超声波提取

Abstract: Objective By optimizing extraction conditions,purification conditions and instrument conditions,to establish a high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS） method for the simultaneous determination of 20 alkaloids in three cosmetic substrates. Methods The sample was extracted by ultrasonic with acetonitrile-water（7 ∶ 3,V/V） mixed solvent containing 1% formic acid,purified by QuEChERS technology,separated by Waters XBrige^TM C₁₈ liquid chromatography column,detected by multiple reaction monitoring（MRM） in positive mode of electric spray ion source（ESI）,and quantified by external standard method. Results The 20 alkaloids had a good linear relationship in the three cosmetics substrates（r>0.996）. The limits of detection and quantitation were 0.015-1.500 μg/kg and 0.049-4.900 μg/kg respectively. The average recoveries of 20 alkaloids were 64.0%-122.0%,and the relative standard deviations were 0.8%-13.3%. Conclusion The method is simple and easy to operate,with high sensitivity,good repeatability and accurate quantification,and is suitable for the detection and safety evaluation of various alkaloids in cosmetics.

Key words: Cosmetics, Alkaloids, High performance liquid chromatography-tandem mass spectrometry, QuEChERS, Ultrasonic extraction

中图分类号:

R115

朱猛, 赖淼菊, 徐斯琦, 熊艳蓝, 崔淑丹, 李强. QuEChERS-高效液相色谱-串联质谱法同时测定化妆品中20种生物碱. [J]职业与健康, 2024, 40(17): 2346-2351.

ZHU Meng, LAI Miaoju, XU Siqi, XIONG Yanlan, CUI Shudan, LI Qiang. Simultaneous determination of 20 alkaloids in cosmetics by QuEChERS-high performance liquid chromatography-tandem mass spectrometry. [J]OCCUPATION AND HEALTH, 2024, 40(17): 2346-2351.

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