UPLC-Q-TOF快速定性定量筛查1起乌头中毒事件

职业与健康 ›› 2025, Vol. 41 ›› Issue (11): 1477-1482.

UPLC-Q-TOF快速定性定量筛查1起乌头中毒事件

寇金蟾¹, 孙明月¹, 周荣贵², 林佶³

1.大理大学公共卫生学院,云南大理 671000;
2.昆明医科大学公共卫生学院,云南昆明 650500;
3.云南省疾病预防控制中心理化实验室,云南昆明 650032

收稿日期:2024-09-04 修回日期:2024-09-19 发布日期:2025-12-12
通信作者: 林佶,主任技师,E-mail：linji666@163.com
作者简介:寇金蟾,男,在读硕士研究生,研究方向为毒物风险监测。

UPLC-Q-TOF rapid qualitative and quantitative screening for an aconitum poisoning event

KOU Jinchan¹, SUN Mingyue¹, ZHOU Rongui², LIN Ji³

1. School of Public Health, Dali University, Dali, Yunnan 671000, China;
2. School of Public Health, Kunming Medical University, Kunming, Yunnan 650500, China;
3. Physical and Chemical Laboratory, Yunnan Center for Disease Control and Prevention, Kunming, Yunnan 650032, China

Received:2024-09-04 Revised:2024-09-19 Published:2025-12-12
Contact: Corresponding auther：LIN Ji,Chief technician,E-mail：linji666@163,com

摘要/Abstract

摘要： 目的对云南省石林县发生的1起群体疑似乌头中毒事件进行流行病学调查并对采集的样品进行乌头碱类化合物的快速定性定量检测。方法 2023年12月27日云南省石林县发生1起群体食物中毒事件,2024年1月2日云南省疾病预防控制中心接收样品并通过超声提取法提取样品中乌头碱类化合物,运用超高效液相色谱串联质谱仪在正模式下选取 C₁₈（2.1 mm×100 mm,1.8 μm）色谱柱进行物质分离,选择乙腈（B）、5 mmoL/L乙酸铵-0.1%甲酸水溶液（A）为梯度洗脱流动相,柱温40 ℃,流速0.3 mL/min,利用各物质精确分子量与二级质谱对食用剩余食物、血液、尿液进行快速筛查。结果在浓度范围5.0~500 ng/mL内,13种标准线性相关系数r均>0.993,稳定性相对标准偏差（relative standard deviation,RSD）≤2.44%, 3种样品加标条件下回收率在75.4%~111.9%,精密度RSD≤2.88%。在剩余食物中检测出苯甲酰次乌头原碱、苯甲酰新乌头原碱、苯甲酰乌头原碱、滇乌头碱、草乌甲素,含量分别为0.21、0.33、0.60、0.20和5.31 mg/kg。结论本次中毒事件是因过量食用乌头炖汤所致的乌头碱中毒。该检测方法灵敏度高、平稳性好,适用于乌头植物毒素检测。

关键词: 超高效液相色谱-四级杆-飞行时间质谱法, 快速筛查, 乌头碱中毒

Abstract: Objective To conduct an epidemiological investigation on a suspected case of aconitine poisoning in Shilin County of Yunnan Province,and to perform rapid qualitative and quantitative detection of aconitine compounds in the collected samples. Methods On December 27,2023,a mass food poisoning incident occurred in Shilin County of Yunnan Province,and on January 2,2024,the Yunnan Center for Disease Control and Prevention received the samples and extracted the aconitine compounds in the samples by ultrasonic extraction,and used ultra-performance liquid chromatography tandem mass spectrometry to select C₁₈（2.1 mm×100 mm,1.8 μm） chromatography column in positive mode for substance separation. Methyl cyanide（B） and 5 mmol/L ammonium acetate-0.1% methane acid water（A） were selected as gradient elution mobile phases,with a column temperature of 40 ℃ and a flow rate of 0.3 mL/min. The precise molecular weight and secondary mass spectrometry of each substance were used for rapid screening of leftover food,blood,and urine. Results In the concentration range of 5.0-500 ng/mL,the linear correlation coefficients of 13 standards were all greater than 0.993,and the relative standard deviation（RSD） of stability was ≤2.44%. The recovery rates of the three samples under spiked conditions were between 75.4%-111.9%,and the RSD of precision was ≤2.88%. Benzoyl hypoaconitine,benzoyl neoaconitine,benzoyl aconitine,Yunnan aconitine and oxaconitin were detected in the leftover meals,with concentrations of 0.21,0.33,0.60,0.20,and 5.31 mg/kg,respectively. Conclusion The poisoning incident was caused by excessive consumption of aconitine stew soup. This detection method has high sensitivity and good stability,and is suitable for the detection of toxins in aconitum plants.

Key words: Ultra-performance liquid chromatography-quadrupole-time-of-flight, Rapid screening, Aconitine poisoning

寇金蟾, 孙明月, 周荣贵, 林佶. UPLC-Q-TOF快速定性定量筛查1起乌头中毒事件. [J]职业与健康, 2025, 41(11): 1477-1482.

KOU Jinchan, SUN Mingyue, ZHOU Rongui, LIN Ji. UPLC-Q-TOF rapid qualitative and quantitative screening for an aconitum poisoning event. [J]OCCUPATION AND HEALTH, 2025, 41(11): 1477-1482.

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