职业性苯乙烯暴露对白血病细胞株HL-60增殖、侵袭、凋亡及细胞周期的影响

职业与健康 ›› 2025, Vol. 41 ›› Issue (11): 1483-1488.

职业性苯乙烯暴露对白血病细胞株HL-60增殖、侵袭、凋亡及细胞周期的影响

阳进

眉山市人民医院儿科,四川眉山 620010

收稿日期:2024-08-02 修回日期:2024-08-12 发布日期:2025-12-12
作者简介:阳进,女,副主任医师,主要从事儿童白血病治疗相关工作。
基金资助:
眉山市科技计划项目（2021108）

Effects of occupational exposure to styrene on proliferation,invasion,apoptosis and cell cycle of leukemia cell line HL-60

YANG Jin

Department of Pediatrics, Meishan People's Hospital, Meishan, Sichuan 620010, China

Received:2024-08-02 Revised:2024-08-12 Published:2025-12-12

摘要/Abstract

摘要： 目的基于miR-126-CSE1L通路探讨职业性苯乙烯暴露对白血病细胞株HL-60增殖侵袭凋亡及细胞周期的影响。方法将对数生长期的白血病细胞株HL-60分别暴露于0、10、20、40 nmoL/mL 的苯乙烯溶液培养72 h后,用CCK-8试剂盒、结晶紫染色测定细胞活性、细胞集落水平,流式细胞仪测定细胞周期及细胞凋亡水平,Transwell实验测定细胞侵袭水平,逆转录聚合酶链式反应及蛋白印记法测定细胞miR-126、CSE1L水平。结果 10、20、40 nmoL/mL染毒剂量的苯乙烯分别处理白血病细胞株HL-60后存活率、细胞集落、侵袭、CSE1L mRNA蛋白表达水平（0.68±0.18 vs 1.18±0.17、1.51±0.15、2.75±0.11,0.26±0.12 vs 0.50±0.11、0.79±0.14、1.24±0.18）明显高于对照组,G2/M期、凋亡率、miR-126表达水平（2.97±0.20 vs 2.24±0.19、1.65±0.14、0.85±0.13）明显低于对照组,差异均有统计学意义（均P<0.05）。苯乙烯10、20、40 nmoL/mL染毒剂量组存活率、细胞集落、侵袭、CSE1L mRNA蛋白表达水平（1.18±0.17、1.51±0.15、2.75±0.11,0.50±0.11、0.79±0.14、1.24±0.18）逐渐升高,G2/M期、凋亡率、miR-126表达水平（2.24±0.19、1.65±0.14、0.85±0.13）逐渐降低,苯乙烯呈剂量依赖性增加了各组细胞存活率、细胞集落、G2/M期、侵袭、CSE1L mRNA蛋白表达水平,降低了各组G2/M期、凋亡率、miR-126表达水平,差异均有统计学意义（均P<0.05）。结论苯乙烯能促进白血病细胞株HL-60增殖、侵袭,抑制凋亡,诱导细胞周期阻滞。其机制可能与苯乙烯抑制白血病细胞株HL-60 miR-126水平,进而靶向上调CSE1L表达有关。

关键词: 苯乙烯, miR-126, CSE1L, 白血病, 增殖, 侵袭, 凋亡, 细胞周期

Abstract: Objective To explore the effect of occupational exposure to styrene on the proliferation,invasion,apoptosis and cell cycle of leukemia cell line HL-60 based on the miR-126-CSE1L pathway. Methods The leukemia cell line HL-60 in the logarithmic growth phase was exposed to styrene solutions of 0,10,20,and 40 nmoL/mL for 72 h. After culture,the cell viability and cell colony levels were measured using CCK-8 kit and crystal violet staining. Flow cytometry was used to measure cell cycle and apoptosis levels,Transwell assay was used to measure cell invasion levels,and RT-PCR and western blot methods were used to measure cellular miR-126 and CSE1L levels. Results After the leukemia cell line HL-60 was treated with styrene at doses of 10,20,and 40 nmoL/mL,the survival rate,cell colony,invasion,and CSE1L mRNA protein expression levels（0.68±0.18 vs 1.18±0.17,1.51±0.15,2.75±0.11;0.26±0.12 vs 0.50±0.11,0.79±0.14,1.24±0.18） were significantly higher than those in the control group,G2/M phase,apoptosis rate,and miR-126 expression level（2.97±0.20 vs 2.24±0.19,1.65±0.14,0.85±0.13） were significantly lower than those in the control group,and the differences were statistically significant（all P<0.05）. The survival rate,cell colony,invasion,and CSE1L mRNA protein expression levels（1.18±0.17,1.51±0.15,2.75±0.11,0.50±0.11,0.79±0.14,1.24±0.18） gradually increased in the styrene exposure dose groups of 10,20,and 40 nmoL/mL,while the G2/M phase,apoptosis rate,and miR-126 expression levels（2.24±0.19,1.65±0.14,0.85±0.13） gradually decreased. Styrene increased the survival rate,cell colonies,G2/M phase,invasion,and CSE1L mRNA protein expression levels in each group in a dose-dependent manner,while reduced G2/M phase,apoptosis rate,and miR-126 expression levels in each group,and the differences were statistically significant（all P<0.05）. Conclusion Styrene can promote the proliferation and invasion of leukemia cell line HL-60,inhibit apoptosis,and induce cell cycle arrest. The mechanism may be related to the inhibition of miR-126 levels in leukemia cell line HL-60 by styrene,which in turn targets the up-regulation of CSE1L expression.

Key words: Styrene, miR-126, CSE1L, Leukemia, Proliferation, Invasion, Apoptosis, Cell cycle

阳进. 职业性苯乙烯暴露对白血病细胞株HL-60增殖、侵袭、凋亡及细胞周期的影响. [J]职业与健康, 2025, 41(11): 1483-1488.

YANG Jin. Effects of occupational exposure to styrene on proliferation,invasion,apoptosis and cell cycle of leukemia cell line HL-60. [J]OCCUPATION AND HEALTH, 2025, 41(11): 1483-1488.

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