水体中20种塑化剂的液液萃取-气相色谱-质谱内标同时测定法

职业与健康 ›› 2025, Vol. 41 ›› Issue (12): 1606-1610.

水体中20种塑化剂的液液萃取-气相色谱-质谱内标同时测定法

曲良娇, 张艳, 梁素丹, 凌莉, 黄文燕

珠海市疾病预防控制中心理化检验所,广东珠海 519000

收稿日期:2025-03-02 修回日期:2025-03-10 出版日期:2025-06-15 发布日期:2025-12-11
通信作者: 黄文燕,主任技师,E-mail：jiaohuster2024@163.com
作者简介:曲良娇,女,主管技师,主要从事水质与食品理化检测工作。
基金资助:
珠海市社会发展领域科技计划项目（2220004000115）

Simultaneous determination of twenty plasticizers in water by liquid-liquid extraction-gas chromatography-mass spectrometry internal standard method

QU Liangjiao, ZHANG Yan, LIANG Sudan, LING Li, HUANG Wenyan

Department of Physical and Chemical Test, Zhuhai Center for Disease Control and Prevention, Zhuhai, Guangdong 519000, China

Received:2025-03-02 Revised:2025-03-10 Online:2025-06-15 Published:2025-12-11
Contact: HUANG Wenyan,Chief technician,E-mail：jiaohuster2024@163.com

摘要/Abstract

摘要： 目的建立同时测定水体中20种塑化剂的液液萃取-气相色谱-质谱内标法。方法采用10 mL二氯甲烷直接萃取250 mL水样,浓缩后经DB-5MS UI色谱柱（30 m×0.250 mm×0.25 μm）分离,在气相色谱-质谱联用仪（gas chromatography-mass spectrometry,GC-MS）的选择性离子监测（selective ion monitoring,SIM）模式下分析,以保留时间和特征离子的相对丰度定性,内标法定量。结果 20种塑化剂均得到良好分离,在0.10~15.00 μg/mL范围内相关系数（r）均>0.995,检出限在0.004~0.34 μg/L之间,定量限在0.01~1.15 μg/L之间,回收率在63.7%~109.0%之间,相对标准偏差（RSD）在0.87%~11.10%。结论该方法简便、灵敏、准确,适用于水体中多种塑化剂的同时检测,可为水体中多种塑化剂的同时检测提供技术参考。

关键词: 气相色谱-质谱法, 液液萃取, 塑化剂, 水体

Abstract: Objective To establish a liquid-liquid extraction-gas chromatography-mass spectrometry internal standard method for the simultaneous determination of twenty plasticizers in water. Methods Totally 250 mL water samples were extracted directly with 10 mL dichloromethane,concentrated and separated by DB-5MS UI column（30 m×0.250 mm×0.25 μm）,and then analyzed by selective ion monitoring（SIM） mode of gas chromatography-mass spectrometry（GC-MS）. Qualitative analysis was performed by retention time and relative abundance of characteristic ions,and quantitative analysis was performed by the internal standard method. Results Twenty plasticizers were effectively separated and showed good linear relationship（r>0.995） in the range of 0.10-15.00 μg/mL. The detection limits and quantitative limits of the method were 0.004-0.34 μg/L and 0.01-1.15 μg/L. The recoveries and relative standard deviations were 63.7%-109.0% and 0.87%-11.10%. Conclusion The method is simple,sensitive and accurate,and is suitable for simultaneous detection of multiple plasticizers in water. It can provide technical reference for simultaneous detection of multiple plasticizers in water.

Key words: Gas chromatography-mass spectrometry, Liquid-liquid extraction, Plasticizers, Water

中图分类号:

R115

曲良娇, 张艳, 梁素丹, 凌莉, 黄文燕. 水体中20种塑化剂的液液萃取-气相色谱-质谱内标同时测定法. [J]职业与健康, 2025, 41(12): 1606-1610.

QU Liangjiao, ZHANG Yan, LIANG Sudan, LING Li, HUANG Wenyan. Simultaneous determination of twenty plasticizers in water by liquid-liquid extraction-gas chromatography-mass spectrometry internal standard method. [J]OCCUPATION AND HEALTH, 2025, 41(12): 1606-1610.

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