2015—2022年彭州市食源性致病菌中沙门菌血清型分布、分子分型及耐药情况分析

职业与健康 ›› 2025, Vol. 41 ›› Issue (2): 172-176.

2015—2022年彭州市食源性致病菌中沙门菌血清型分布、分子分型及耐药情况分析

林惠¹, 袁齐武², 张凤菊³

1.彭州市疾病预防控制中心检验科,四川成都 611930;
2.成都市疾病预防控制中心微生物检验科,四川成都 610041;
3.四川省农业科学院农产品加工研究所发酵食品研究中心,四川成都 610066

收稿日期:2024-05-05 修回日期:2024-05-14 出版日期:2025-01-15 发布日期:2025-12-11
作者简介:林惠,女,主管技师,主要从事微生物检验工作。
基金资助:
四川省科技计划项目（2023YFS0399）

Analysis of serotype distribution,molecular typing and drug resistance of Salmonella in foodborne pathogens in Pengzhou City from 2015 to 2022

LIN Hui¹, YUAN Qiwu², ZHANG Fengju³

1. Department of Clinical Laboratory, Pengzhou Center for Disease Control and Prevention, Chengdu, Sichuan 611930, China;
2. Department of Microbiology Laboratory, Chengdu Center for Disease Control and Prevention, Chengdu, Sichuan 610041, China;
3. Research Center for fermented food, Institute of Agro-products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu, Sichaun610066, China

Received:2024-05-05 Revised:2024-05-14 Online:2025-01-15 Published:2025-12-11

摘要/Abstract

摘要： 目的了解2015—2022年彭州市食源性致病菌中沙门菌的血清型分布、分子分型及耐药情况,为沙门菌感染的合理用药及今后食源性疾病暴发和溯源提供参考依据。方法收集2015—2022年彭州市两家食源性疾病监测哨点医院上送的食源性疾病分离培养菌株,参照GB 4789.4及诊断血清说明书进行沙门菌的复核鉴定和血清型分型,随机选择部分样本进行药敏试验和脉冲场凝胶电泳（plused-field gel electrophoresis,PFGE）分型。结果共收集260株食源性疾病病例沙门菌标本,鉴定出20种血清型,其中鼠伤寒沙门菌（71.15%）和肠炎沙门菌（17.69%）占据主要优势;随机选择的50株沙门菌分成了44个PFGE带型,呈多样性,有成簇带型出现,出现相同带型的有5种,每种带型包含2~3株菌,聚类相似度为27.18%~100.00%;随机选择的40株沙门菌17种抗生素药敏试验,对氨苄西林、链霉素、四环素、萘啶酸耐药率较高,对阿奇霉素和阿米卡星敏感率为100.00%,对厄他培南、头孢他啶/阿维巴坦、美罗培南的敏感率均>90.00%。有4株对所有的抗生素敏感,有7株对1种或2种抗生素耐药,其余29株有不同程度的多重耐药,多重耐药率为72.50%,以耐4种和5种抗生素菌株较多,最高可对9种抗生素同时耐药。结论 2015—2022年彭州市食源性致病菌中沙门菌主要血清型为鼠伤寒沙门菌和肠炎沙门菌,沙门菌PFGE带型呈多样性,存在小范围流行的风险,多重耐药现象严重。

关键词: 沙门菌, 血清型分型, 药敏实验, 脉冲场凝胶电泳

Abstract: Objective To investigate the serotype distribution,molecular typing and drug resistance of salmonella in foodborne pathogens in Pengzhou City from 2015 to 2022,and to provide scientific theoretical basis for rational drug use of salmonella infection and the outbreak and tracing of foodborne diseases in the future. Methods Food-borne disease isolates were collected from two food-borne disease surveillance sentinel hospitals in Pengzhou City from 2015 to 2022. Re-examination identification and serological typing of salmonella were carried out according to GB4789.4 and the instructions for diagnostic serums. Drug sensitivity tests and pluse-field gel electrophoresis（PFGE） were used to randomly select certain samples for typing. Results A total of 260 Salmonella isolates were collected from food-borne disease cases and 20 serotypes were identified. Among them,Salmonella typhimurium（71.15%） and Salmonella enteritidis（17.69%） were dominant. The 50 randomly selected Salmonella strains were divided into 44 PFGE band types,which showed diversity,and cluster band types appeared,there were 5 kinds of the same band types,each band type contained 2-3 strains,and the cluster similarity was 27.18%-100.00%. The 40 randomly selected Salmonella strains showed high resistance to ampicillin,streptomycin,tetracycline and nalidixic acid,100.00% sensitivity to azithromycin and amicacin,and >90.00% sensitivity to ertapenem,ceftazidime/avibactam and meropenem. 4 strains were sensitive to all antibiotics,7 strains were resistant to 1 or 2 antibiotics,and the remaining 29 strains had different degrees of multiple drug resistance,the multiple drug resistance rate was 72.50%,and the most resistant strains were resistant to 4 and 5 antibiotics,the highest was resistant to 9 antibiotics at the same time. Conclusions The main serotypes of salmonella in food-borne pathogens in Pengzhou from 2015 to 2022 are Salmonella typhimurium and Salmonella enteritidis.The PFGE band types of Salmonella are diverse,there is a risk of small epidemic,and the phenomenon of multiple drug resistance is serious.

Key words: Salmonella, Serotyping, Drug sensitivity test, Pluse-field gel electrophoresis

中图分类号:

R117

林惠, 袁齐武, 张凤菊. 2015—2022年彭州市食源性致病菌中沙门菌血清型分布、分子分型及耐药情况分析. [J]职业与健康, 2025, 41(2): 172-176.

LIN Hui, YUAN Qiwu, ZHANG Fengju. Analysis of serotype distribution,molecular typing and drug resistance of Salmonella in foodborne pathogens in Pengzhou City from 2015 to 2022. [J]OCCUPATION AND HEALTH, 2025, 41(2): 172-176.

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