槲皮素对口腔正常细胞株和癌细胞株的不同影响及抗氧化机制研究

职业与健康 ›› 2025, Vol. 41 ›› Issue (6): 763-767.

槲皮素对口腔正常细胞株和癌细胞株的不同影响及抗氧化机制研究

周晓

山东医学高等专科学校基础部病理学教研室,山东临沂 276000

收稿日期:2024-09-12 修回日期:2025-02-26 出版日期:2025-03-15 发布日期:2025-12-16
作者简介:周晓,男,副教授,主要从事病理学与病理生理学的研究与教学工作。

Study on the different effects of quercetin on normal oral cell lines and cancer cell lines and their antioxidant mechanisms

ZHOU Xiao

Pathology Teaching and Research Office,Basic Department,Shandong Medical College,Linyi,Shandong 276000,China

Received:2024-09-12 Revised:2025-02-26 Online:2025-03-15 Published:2025-12-16

摘要/Abstract

摘要： 目的观察槲皮素对人口腔正常上皮细胞和口腔癌细胞增殖、迁移和损伤的影响并探讨其机制。方法体外培养DOK和HSC-4细胞株,将两种细胞均分为对照组和槲皮素（50 μmol/L）处理组,HSC-4细胞株增设槲皮素（50 μmol/L）、H₂O₂（1 μmol/L）组。观察DOK和HSC-4细胞株对槲皮素干预的反应,比色法检测各组培养液乳酸脱氢酶（lactate dehydrogenase,LHD）含量,平板集落形成实验检测各组集落形成率,细胞侵袭实验检测各组细胞迁移能力、Annexin-V-FITC-PI双染流式细胞仪检测各组细胞死亡率。探讨槲皮素发挥抗口腔癌作用的可能机制,比色法检测DOK、HSC-4各组细胞内活性氧（reactive oxygen species,ROS）水平。结果人口腔正常上皮细胞DOK槲皮素处理组与对照组比较,各项检测指标差异均无统计学意义（均P>0.05）。人口腔癌细胞HSC-4槲皮素处理组（0.37±0.047,0.302±0.035,56±9.6,89±10.0, 191±22）、槲皮素+H₂O₂组（0.25±0.040,0.199±0.015,72±11.0,112±12.0,220±19）、对照组（0.15±0.031,0.113±0.020,85±8.6, 135±15.0,263±25）两两比较,损伤程度、死亡率、集落形成率（%）、侵袭能力与细胞内ROS水平差异均有统计学意义（均P<0.05）。结论槲皮素干预对人口腔正常上皮细胞DOK无显著影响,但对人口腔癌细胞HSC-4具有抑制增殖和迁移、促进损伤和提高死亡率的作用,其作用机制可能与槲皮素降低了HSC-4细胞内的ROS水平有关。

关键词: 槲皮素, 口腔正常细胞, 口腔癌细胞, 活性氧

Abstract: Objective To observe the effects of quercetin on the proliferation,migration,and damage of normal human oral epithelial cells and oral cancer cells ,and explore its mechanism. Methods DOK and HSC-4 cell lines were cultured in vitro,and two types of cells both were divided into a control group and a quercetin（50 μmol/L） treated group.HSC-4 cell line added quercetin（50 μmol/L） group and H₂O₂（10 μmol/L） group. The response of DOK and HSC-4 cell lines to quercetin intervention was observed,lactate dehydrogenase（LDH） content of each group's culture medium was detected by colorimetric method,colony formation rate of each group was detected by plate colony formation assay,cell migration ability of each group was detected by cell invasion assay,and cell mortality rate of each group was detected by Annexin-V-FITC-PI dual staining flow cytometry. To explore the possible mechanism of quercetin's anti oral cancer effect,intracellular reactive oxygen species（ROS） levels in DOK and HSC-4 groups was detected by colorimetric method. Results There were no statistically significant differences in various detection indicators between the quercetin treated group and the control group of normal oral epithelial cells DOK（all P>0.05）. There were statistically significant differences in the degree of damage,mortality rate,colony formation rate（%）,invasion ability,and intracellular ROS levels between the HSC-4 oral cancer cells of quercetin treated group,quercetin+H₂O₂ group,and control group（all P<0.05）. Conclusion Quercetin intervention has no significant effect on the proliferation,migration,and apoptosis of normal human oral epithelial cells DOK,but it has inhibitory effects on proliferation and migration,promotes damage,and increases mortality in human oral cancer cell line HSC-4. Its mechanism of action may be related to the reduction of ROS levels in HSC-4 cells by quercetin.

Key words: Quercetin, Normal oral cells, Oral cancer cells, Reactive oxygen species

中图分类号:

R329.21

周晓. 槲皮素对口腔正常细胞株和癌细胞株的不同影响及抗氧化机制研究. [J]职业与健康, 2025, 41(6): 763-767.

ZHOU Xiao. Study on the different effects of quercetin on normal oral cell lines and cancer cell lines and their antioxidant mechanisms. [J]OCCUPATION AND HEALTH, 2025, 41(6): 763-767.

参考文献

[1] KAM L C,NIEMAN D C,SHA W,et al.Dose-response to 3 months of quercetin-containing supplements on metabolite and quercetin conjugate profile in adults[J].Br J Nutr,2013,109:1923-1933.
[2] 赵梓亦,熊小明,谢雨鹏,等.槲皮素通过雌激素受体下调长非编码RNA MALAT-1并发挥抗乳腺癌的作用机制[J].中国药理学通报,2024,40(3):499-505
[3] 池周,张豪,蔡玉节,等.槲皮素通过PI3K/AKT信号通路抑制奥沙利铂耐药胃癌细胞迁移[J].实用药物与临床,2024,27(2):81-85.
[4] 陈林金,谢应玉,陈丹,等.基于JAK/STAT信号通路探讨槲皮素通过调控IL-6对结肠癌模型大鼠的保护机制[J].中国老年学杂志,2024,44(3):646-650.
[5] 潘小娟,史晓光,汪唐顺,等.基于肉瘤基因信号通路探讨槲皮素对乳腺癌MCF-7细胞的作用机制[J].世界中医药,2023,18(20):2892-2898.
[6] TANG S M,DENG X T,ZHOU J,et al.Pharmacological basis and new insights of quercetin action in respect to its anti-cancer effects[J].Biomed Pharmacother,2020,121:109604.
[7] 付连港,曾建国.槲皮素体内的抗氧化活性研究及在医药领域的应用[J].工业微生物,2024,54(1):8-10.
[8] 肖兰飞,高继萍,闫晓如,等.槲皮素对口腔癌Tca-8113细胞增殖、迁移和侵袭的影响及PI3K/AKT信号通路的调控作用[J].中国比较医学杂志,2021,31(6):30-34.
[9] AMORIM R,PINHEIRO C,GONCALVES M V,et al.Monocarboxylate transport inhibition potentiates the cytotoxic effect of 5-fluorouracil in colorectal cancer cells[J].Cancer Lett,2015,365(1):68-78.
[10] HE C,LU X,LI J,et al.The effect of quercetin on cervical cancer cells as determined by inducing tumor endoplasmic reticulum stress and apoptosis and its mechanism of action[J].Am J Transl Res,2021,13(5):5240-5247.
[11] ZERIN T,KIM Y S,HONG S Y,et al.Quercetin reduces oxidative damage induced by paraquat via modulatingexpression of antioxidant genes in A549 cells[J].J Appl Toxicol,2013,33(12):1460-1467.
[12] KOUNDOUROS N,POULOGIANNIS G.Phosphoinositide 3-Kinase/Akt signaling and redox metabolism in cancer[J].Front Oncol,2018, 8:160.
[13] CHUA P J,YIP G W,BAY B H.Cell cycle arrest induced by hydrogen peroxide is associated with modulation of oxidative stress related genes in breast cancer cells experimental biology and medicine[J].Exp Biol Med(Maywood),2009,234(9):1086-1094.
[14] PARK I J,HWANG J T,KIM Y M,et al.Differential modulation of AMPK signaling pathways by low or high levels of exogenous reactive oxygen species in colon cancer cells[J].Ann N Y Acad Sci J,2006, 1091:102-109.
[15] MAURYA A K,VINAYAK M.PI-103 and quercetin attenuate PI3K-AKT signaling pathway in T-cell lymphoma exposed to hydrogen peroxide[J].PLoS One,2016,11(8):e0160686.