温度和PM2.5暴露对小鼠肺泡巨噬细胞影响的实验研究

职业与健康 ›› 2025, Vol. 41 ›› Issue (7): 899-903.

温度和PM_2.5暴露对小鼠肺泡巨噬细胞影响的实验研究

宋全全¹, 柳淑琴², 孙武¹, 王秀珍¹, 李倩¹, 张书余³

1.广元市精神卫生中心公共卫生科,四川广元 628000;
2.广元市中医医院呼吸科,四川广元 628000;
3.河北省气象与生态环境重点实验室,河北石家庄 050021

收稿日期:2024-06-25 修回日期:2024-07-22 出版日期:2025-04-01 发布日期:2025-12-17
通信作者: 孙武,主任医师,E-mail：544483849@qq.com
作者简介:宋全全,男,主治医师,主要从事环境毒理学与劳动卫生研究工作。
基金资助:
国家自然科学基金（41375121）; 河北省重点研究计划资助项目（18273705D）; 广元市科技局重点研发项目（23ZDYF0085）

Experimental study on the effect of temperature and PM_2.5 exposure on alveolar macrophages in mice

SONG Quanquan¹, LIU Shuqin², SUN Wu¹, WANG Xiuzhen¹, LI qian¹, ZHANG Shuyu³

1. Department of Public Health,Guangyuan Mental Health Center,Guangyuan,Sichuan 628000,China;
2. Department of Respiratory,Guangyuan Hospital of Traditional Chinese Medicine,Guangyuan,Sichuan 628000,China;
3. Hebei Provincial Key Laboratory of Meteorology and Ecological Environment,Shijiazhuang,Hebei 050021,China

Received:2024-06-25 Revised:2024-07-22 Online:2025-04-01 Published:2025-12-17
Contact: SUN Wu,Chief physician,E-mail：544483849@qq.com

摘要/Abstract

摘要： 目的探究不同温度与细颗粒物（fine particulate matter,PM_2.5）刺激对小鼠肺泡巨噬细胞（alveolar macrophages,AMs）的毒性影响及其机制。方法提取小鼠AMs,分别在27、32、37、42 ℃温度下培养,每个温度组分别给予不同浓度PM_2.5处理,染毒12 h后检测小鼠AMs相关指标水平。结果相同染毒剂量下,常温组细胞相对存活率和吞噬功能最高,温度升高或者降低,细胞吞噬功能均下降。相同温度下,PM_2.5处理组细胞吞噬能力显著低于对照组,且PM_2.5浓度越高细胞相对存活率越低,毒性作用越强。与对照组和常温组相比,低温与较高浓度PM_2.5联合染毒可引起白细胞介素-6（interleukin-6,IL-6）、C反应蛋（C-reactive protein,CRP）含量显著升高（P<0.05）,而与常温组相比,各组巨噬细胞炎症蛋白1α（macrophage inflammatory protein 1α,MIP-1α）水平均无显著增加（P>0.05）。随着PM_2.5染毒剂量的增加小鼠AMs中超氧化物歧化酶（superoxide dismutase,SOD）活性显著降低和丙二醛（malondialdehyde,MDA）水平显著升高（P<0.05）,此外,温差越大PM_2.5浓度越高,对小鼠AMs的SOD和MDA的影响越明显。结论不同温度与PM_2.5染毒均会对小鼠AMs细胞产生毒性作用,其机制可能通过诱发细胞炎症反应和氧化应激损伤使其吞噬功能下降,且低温和高剂量PM_2.5暴露对AMs的影响上存在交互作用。

关键词: 温度, 细颗粒物, 肺泡巨噬细胞, 交互作用

Abstract: Objective To investigate the toxic effects and mechanisms of different temperatures and fine particulate matter（PM_2.5） exposure on alveolar macrophages（AMs）. Methods The AMs of mice were extracted and cultured at 27,32,37 and 42 ℃,respectively,each of which was treated with different concentrations of PM_2.5. After 12 hours of exposure,the levels of AMs relative indicators in mice were detected. Results At the same exposure dose,the relative survival rate and phagocytic function of cells in the normal temperature group were the highest,and the phagocytic function of cells decreased with increasing or decreasing temperature. At the same temperature,the phagocytic ability of cells in the PM_2.5 treatment group was significantly lower than that in the control group,and the higher the PM_2.5 concentration,the lower the relative survival rate of cells and the stronger the toxic effect. A low temperature combined higher concentrations of PM_2.5 exposure could cause the levels of interleukin-6（IL-6） and C-reactive protein（CRP） increased significantly compared with the control group and the normal temperature group（P<0.05）. However, compared with the normal temperature group,there were no significant increase in the levels of macrophage inflammatory protein 1α（MIP-1α） in each group（all P>0.05）. With the increase of PM_2.5 exposure dose,the activity of superoxide dismutase（SOD） in mouse AMs significantly decreased and the level of malondialdehyde（MDA） significantly increased（both P<0.05）. In addition,the larger the temperature difference,the higher the PM_2.5 concentration,and the more obvious the effect on SOD and MDA in mouse AMs. Conclusion Different temperatures and exposure to PM_2.5 can have toxic effects on mouse AMs cells,which may be mediated by inducing cellular inflammatory responses and oxidative stress damage,leading to a decrease in phagocytic function. Additionally,there is an interactive effect between low temperature and high-dose PM_2.5 exposure on AMs.

Key words: Temperature, Fine particulate matter, Alveolar macrophages, Interaction

中图分类号:

R114

宋全全, 柳淑琴, 孙武, 王秀珍, 李倩, 张书余. 温度和PM_2.5暴露对小鼠肺泡巨噬细胞影响的实验研究. [J]职业与健康, 2025, 41(7): 899-903.

SONG Quanquan, LIU Shuqin, SUN Wu, WANG Xiuzhen, LI qian, ZHANG Shuyu. Experimental study on the effect of temperature and PM_2.5 exposure on alveolar macrophages in mice. [J]OCCUPATION AND HEALTH, 2025, 41(7): 899-903.

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