关键词: 果蔬, 农药残留, QuEChERS, 气相色谱-串联质谱

Abstract:

Objective To establish a quick，easy，cheap，effective，rugged and safe（QuEChERS） combined with gas chromatography-tandem mass spectrometry method for the determination of 104 pesticide residues in fruits and vegetables. Methods The samples were extracted with acetonitrile and QuEChERS extraction salt packets，and then purified with anhydrous magnesium sulfate and primary secondary amine（PSA）. Separation was performed on an HP-5MS UI column，and detection was carried out using dynamic multiple reaction monitoring（dMRM） mode. Quantification was achieved by external standard calibration with matrix-matched standards. Results Under the refined experimental conditions，the 104 pesticides exhibited strong linearity across a concentration range of 10 to 500 μg/L，with correlation coefficients greater than 0.995. The mean recovery rates at various concentration levels fell between 71.18% and 123.43%，and the relative standard deviations were within the range of 0.613% to 9.523%（n=6）. The limits of detection varied from 0.000 012 to 0.002 2 mg/kg，while the limits of quantification ranged from 0.000 041 to 0.007 2 mg/kg. Conclusion The method has the advantages of good selectivity，high accuracy，and strong sensitivity，and can meet the requirements for detection and analysis of diverse pesticide residues in fruit and vegetable samples.

Key words: Fruits and vegetables, Pesticide residues, Quick,easy,cheap,effective,rugged and safe, Gas chromatography-tandem mass spectrometry