1-硝基芘通过自噬通路对秀丽隐杆线虫寿命的影响研究

职业与健康 ›› 2024, Vol. 40 ›› Issue (2): 195-199.

1-硝基芘通过自噬通路对秀丽隐杆线虫寿命的影响研究

付玉聪^1a, 王萌^1b, 刘盛杰², 赵理乐², 张绪梅^1b, 张利文^1a

1.天津医科大学公共卫生学院 a劳动卫生与环境卫生学系, b营养与食品卫生学系,天津 300070;
2.天津市西青医院脑系科,天津 300380

收稿日期:2023-05-15 修回日期:2023-06-19 出版日期:2024-01-15 发布日期:2026-03-11
通信作者: 张利文,副教授,E-mail：zhangliwen@tmu.edu.cn
作者简介:付玉聪,男,在读硕士研究生,研究方向为劳动卫生与环境卫生。
基金资助:
国家环境保护机动车污染控制与模拟重点实验室开放基金（VECS2022K04）;天津市西青医院天津医科大学科联体项目（XQYYKLT202102）

Effects of 1-nitropyrene on lifespan of Caenorhabditis elegans through autophagy pathway

FU Yucong^1a, WANG Meng^1b, LIU Shengjie², ZHAO Lile², ZHANG Xumei^1b, ZHANG Liwen^1a

1. a Department of Labor Health and Environmental Hygiene, b Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University, Tianjin, 300070, China;
2. Cerebral Department, Tianjin Xiqing Hospital, Tianjin, 300380, China

Received:2023-05-15 Revised:2023-06-19 Online:2024-01-15 Published:2026-03-11
Contact: ZHANG Liwen,Associate professor,E-mail：zhangliwen@tmu.edu.cn

摘要/Abstract

摘要： 目的本研究采用秀丽隐杆线虫（简称线虫）模型探讨1-硝基芘（1-nitropyrene,1-NP）对线虫寿命的影响和自噬在寿命影响中的作用。方法在正常培养条件下,研究不同浓度的1-NP（0、10^-4、10^-2、10²、10⁴ ng/L）亚急性干预5天对线虫寿命的影响,观察1-NP对线虫健康相关参数如体长、运动能力和脂褐素和寿命的影响。使用实时定量聚合酶链反应（quantitative real time-polymerase chain reaction,qRT-PCR）检测自噬相关标志基因（bec-1和lgg-1）的表达情况。结果与对照组相比,1-NP可显著降低线虫的寿命,其中 10⁴ ng/L剂量降低寿命效果最明显,降低率为 28.2%。1-NP（10⁴ ng/L）可降低线虫的体长（P<0.05）和自噬关键基因表达水平（P<0.05）,增加线虫体内脂褐素的蓄积（P<0.05）。结论 1-NP可以通过降低线虫体内自噬水平,进而使线虫的寿命缩短。

关键词: 1-硝基芘, 秀丽隐杆线虫, 衰老, 自噬, 脂褐素

Abstract: Objective By using the Caenorhabditis elegans model（hereafter referred to as C. elegans）,to investigate the effects of 1-nitropyrene（1-NP） on the lifespan of C. elegans and the role of autophagy in the impact of lifespan. Methods The effects of subacute exposure（five days） of 1-NP（0,10^-4,10^-2,10²,10⁴ ng/L） on the life span of C. elegans were studied under normal culture conditions. The effects of 1-NP on health indicators（body length,exercise capacity and lipofuscin） and longevity of C. elegans were observed. The expression of autophagy related marker genes（bec-1 and lgg-1） was detected by quantitative real time-polymerase chain reaction（qRT-PCR）. Results Compared with the control group,1-NP could significantly reduce the lifespan of C. elegans,and 10⁴ ng/L group had the most significant effect,with a reduction rate of 28.2%. 1-NP（10⁴ ng/L） decreased the body length of C. elegans（P<0.05） and the expression levels of key autophagy genes（bec-1 and lgg-1）（P<0.05）,increased lipofuscin accumulation in C. elegans（P<0.05）. Conclusion 1-NP can shorten the lifespan of C. elegans by decreasing the autophagy level in C. elegans.

Key words: 1-nitropyrene, Caenorhabditis elegans, Aging, Autophagy, Lipofuscin

中图分类号:

R114

付玉聪, 王萌, 刘盛杰, 赵理乐, 张绪梅, 张利文. 1-硝基芘通过自噬通路对秀丽隐杆线虫寿命的影响研究. [J]职业与健康, 2024, 40(2): 195-199.

FU Yucong, WANG Meng, LIU Shengjie, ZHAO Lile, ZHANG Xumei, ZHANG Liwen. Effects of 1-nitropyrene on lifespan of Caenorhabditis elegans through autophagy pathway. [J]OCCUPATION AND HEALTH, 2024, 40(2): 195-199.

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