天津市市场水产品中副溶血性弧菌的污染情况及毒力基因分布研究

职业与健康 ›› 2025, Vol. 41 ›› Issue (21): 2918-2922.

天津市市场水产品中副溶血性弧菌的污染情况及毒力基因分布研究

宁培勇^1,2, 范小晴^1,2, 邹明^1,2, 罗莎², 卓越^1,2, 李晓燕^1,2

1.天津市传染病病原微生物重点实验室,天津 300011;
2.天津市疾病预防控制中心,天津 300011

收稿日期:2025-08-18 修回日期:2025-09-15 发布日期:2025-12-15
通信作者: 李晓燕,主任技师,E-mail：xiaoyanli1291@163.com
作者简介:宁培勇,男,主任医师,主要从事微生物学检测工作。
基金资助:
天津市自然科学基金项目（No.24JCQNJC00430）

Study on the contamination status and virulence gene distribution of Vibrio parahaemolyticus in seafood sold in Tianjin City

NING Peiyong^1,2, FAN Xiaoqing^1,2, ZOU Ming^1,2, LUO Sha², ZHUO Yue^1,2, LI Xiaoyan^1,2

1. Tianjin Key Laboratory of Pathogenic Microbiology of Infectious Disease,Tianjin 300011,China;
2. Tianjin Center for Disease Control and Prevention,Tianjin 300011,China

Received:2025-08-18 Revised:2025-09-15 Published:2025-12-15
Contact: LI Xiaoyan,Chief technician,E-mail：xiaoyanli1291@163.com

摘要/Abstract

摘要： 目的了解天津市市售水产品中副溶血性弧菌的污染情况及毒力基因分布,并结合全基因组测序技术分析其毒力基因与菌株遗传进化关系。方法 2024年9—10月,采集30份水产品样本,通过细菌学方法分离鉴定副溶血性弧菌,并利用PCR检测其毒力基因不耐热溶血素（tlh）、耐热直接溶血素（tdh）和耐热相关溶血素（trh）。对分离菌株进行全基因组测序,分析其毒力基因谱,并运用生物信息学探讨菌株间的遗传进化关系。结果 30份样本增菌液中,副溶血性弧菌核酸阳性率为36.67%（11/30）,其中tlh基因阳性率为33.33%（10/30）,而tdh与trh基因均为6.67%（2/30）。共分离6株副溶血性弧菌,其核酸及tlh基因阳性率均为20.00%（6/30）,但tdh和trh基因完全缺失。全基因组测序显示,毒力基因主要集中于“粘附”和“效应器传递系统”功能类别。基因组比对表明,分离株类型Ⅲ分泌系统与参考基因组RIMD2210633的同源性为94%~100%,存在一定序列变异。结论天津市市售水产品中副溶血性弧菌的污染率较高。全基因组测序揭示,其毒力基因主要与粘附与效应器传递系统相关,提示这些功能在致病过程中发挥关键作用。T3SS1基因的同源性表明其在菌株进化中较为保守。本研究为流行病学调查及食品安全风险评估提供了分子证据。

关键词: 水产品, 副溶血性弧菌, 毒力基因, 类型Ⅲ分泌系统

Abstract: Objective To investigate the contamination status and virulence gene distribution of Vibrio parahaemolyticus in seafood sold in Tianjin,and to analyze virulence gene and the genetic evolutionary relationships between strains using whole-genome sequencing(WGS). Methods From September to October 2024,a total of 30 seafood samples were collected,and V. parahaemolyticus was isolated and identified by conventional bacteriological methods. Polymerase chain reaction(PCR) was employed to detect the virulence genes(tlh,tdh,and trh). Whole-genome sequencing was performed on the isolated strains to characterize the virulence gene profiles,and bioinformatics tools were used to assess their genetic evolutionary relationships. Results V. parahaemolyticus nucleic acid was detected in 36.67%(11/30) of the 30 enriched samples,with tlh gene positivity in 33.33%(10/30) and tdh and trh genes detected in 6.67%(2/30). Six strains of V. parahaemolyticus were isolated,all of which tested positive for tlh(20.00%,6/30),whereas tdh and trh genes were completely absent. WGS analysis revealed that the virulence genes were primarily associated with adhesion and effector delivery system. Comparative genomic analysis showed that the type Ⅲ secretion system 1(T3SS1) gene in the isolated strains shared 94%-100% homology with the reference strain RIMD2210633,indicating certain sequence variations. Conclusion The prevalence of V. parahaemolyticus in seafood sold in Tianjin is relatively high. WGS analysis indicates that virulence-associated genes are mainly involved in adhesion and effector delivery system,suggesting their crucial role in pathogenesis. The high homology of the T3SS1 gene suggests its evolutionary conservation among strains. This study provides molecular evidence for epidemiological surveillance and food safety risk assessment.

Key words: Seafood, Vibrio parahaemolyticus, Virulence genes, T3SS1

中图分类号:

R155.5

宁培勇, 范小晴, 邹明, 罗莎, 卓越, 李晓燕. 天津市市场水产品中副溶血性弧菌的污染情况及毒力基因分布研究. [J]职业与健康, 2025, 41(21): 2918-2922.

NING Peiyong, FAN Xiaoqing, ZOU Ming, LUO Sha, ZHUO Yue, LI Xiaoyan. Study on the contamination status and virulence gene distribution of Vibrio parahaemolyticus in seafood sold in Tianjin City. [J]OCCUPATION AND HEALTH, 2025, 41(21): 2918-2922.

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