尿中多环芳烃代谢物的超高效液相色谱-串联质谱测定法

职业与健康 ›› 2023, Vol. 39 ›› Issue (10): 1329-1333.

尿中多环芳烃代谢物的超高效液相色谱-串联质谱测定法

王甫¹, 苏凡², 罗兰¹, 秦威振¹

1.深圳市龙华区疾病预防控制中心理化检验科,广东深圳 518109;
2.中山大学公共卫生学院,广东广州 518109

收稿日期:2022-09-06 修回日期:2022-10-17 发布日期:2026-03-13
作者简介:王甫,男,主管技师,主要从事理化检验工作。
基金资助:
深圳市龙华区医疗卫生机构2020年区级科研项目（2020204）

Determination of polycyclic aromatic hydrocarbons metabolites in urine by ultra-performance liquid chromatography tandem mass spectrometry

WANG Fu¹, SU Fan², LUO Lan¹, QIN Wei-zhen¹

1. Physical and Chemical Testing Department,Longhua District Center for Disease Control and Prevention,Shenzhen Guangdong 518109,China;
2. School of Public Health,Sun Yat-sen University,Guangzhou Guangdong 510080,China

Received:2022-09-06 Revised:2022-10-17 Published:2026-03-13

摘要/Abstract

摘要： 目的建立一种尿中12种多环芳烃代谢物的超高效液相色谱-串联质谱测定方法。方法 2022年选取深圳市的健康成人取其尿样经β-葡萄糖醛酸酶水解后,C₁₈固相萃取柱净化富集,氮吹浓缩,经Waters Acquity UPLC HSS T3 C₁₈色谱柱（100 mm×2.1 mm×1.8 μm）分离,在选择反应监测模式下使用UPLC-MS/MS进行检测,内标法定量分析。结果 12种多环芳烃代谢物在0.01~200 ng/mL线性范围良好,相关系数均>0.997 0,检出限为0.002~0.041 ng/mL,定量下限为0.007~0.135 ng/mL,样品加标回收率为88.4%~119.2%。方法批内精密度相对标准偏差（relative standard deviations,RSD）为2.8%~10.3%,批间精密度RSD为3.1%~10.7%。结论该方法操作简便,灵敏度高、精密度高、检出限低,适合用于人体尿液中12种多环芳烃代谢物的测定。

关键词: 多环芳烃代谢物, 尿, 固相萃取, 超高效液相色谱-串联质谱

Abstract: Objective To develop a method for determination of 12 polycyclic aromatic hydrocarbons metabolites in urine by ultra-performance liquid chromatography tandem mass spectrometry. Methods In 2022,the healthy adults from Shenzhen City were selected to collect their urine samples,the samples were hydrolyzesd by β-glucuronidase,purified and enriched by C₁₈ solid phase extraction cartridges,concentrated by nitrogen blowing,separated by Waters Acquity UPLC HSS T3 C₁₈ column（100 mm×2.1 mm×1.8 μm） and finally determined with UPLC-MS/MS under select response monitoring mode,quantified by internal standard method. Results 12 polycyclic aromatic hydrocarbons metabolites have a good linear relationship in range of 0.01-200 ng/mL,the correlation coefficients were above 0.997 0. The limits of detection were 0.002-0.041 ng/mL,the lower limits of quantitation were 0.007-0.135 ng/mL,and the sample spiking recovery rates were 88.4%-119.2%.The relative standard deviations（RSD） of intra batch precision were 2.8%-10.3%,and the RSD of inter batch precision were 3.1%-10.7%. Conclusion This method has the advantages of easy operation,high sensitivity,high precision,low detection limit,and it issuitable for the determination of 12 polycyclic aromatic hydrocarbons metabolites in urine.

Key words: Polycyclic aromatic hydrocarbons metabolites, Urine, Solid phase extraction, Ultra-performance liquid chromatography tandem mass spectrometry

中图分类号:

R115

王甫, 苏凡, 罗兰, 秦威振. 尿中多环芳烃代谢物的超高效液相色谱-串联质谱测定法. [J]职业与健康, 2023, 39(10): 1329-1333.

WANG Fu, SU Fan, LUO Lan, QIN Wei-zhen. Determination of polycyclic aromatic hydrocarbons metabolites in urine by ultra-performance liquid chromatography tandem mass spectrometry. [J]OCCUPATION AND HEALTH, 2023, 39(10): 1329-1333.

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