血清miR-135a-5p、TRPC1水平在职业性哮喘预后评估中的价值

摘要/Abstract

摘要： 目的研究血清微小RNA-135a-5p（microRNA-135a-5p,miR-135a-5p）、瞬时受体电位通道1（transient receptor potential channel 1,TRPC1）在职业性哮喘预后中的预测价值。方法将2019年1月—2021年3月山东颐养健康集团莱芜中心医院收治的96例职业性哮喘患者作为观察组,根据疾病严重程度分为轻度、中度和重度组;根据治疗后1年内随访是否复发分为复发组和未复发组;同期健康检查者92例作为对照组。采用实时荧光定量PCR（quantitative real-time PCR,qRT-PCR）法检测血清miR-135a-5p水平,采用酶联免疫吸附（enzyme linked immunosorbent assay,ELISA）法检测血清TRPC1及气道炎症指标白细胞介素（interleukin,IL）-4、IL-12、肿瘤坏死因子-α（tumor necrosis factor-α,TNF-α）水平,采用肺功能仪检测第1秒用力呼气容积（forced expiratory volume in one second,FEV1）、用力肺活量（forced vital capacity,FVC）、最大呼气流量（peak expiratory flow,PEF）,计算FEV1/FVC;职业性哮喘患者血清miR-135a-5p、TRPC1与肺功能指标及气道炎症指标的相关性采用Pearson相关分析;影响职业性哮喘患者复发的因素采用多因素logistic回归分析;血清miR-135a-5p、TRPC1单独及联合预测职业性哮喘患者复发的价值采用受试者工作特征（receiver operator characteristic,ROC）曲线分析。结果观察组血清miR-135a-5p水平显著低于对照组（P<0.05）,TRPC1水平显著高于对照组（P<0.05）。随疾病严重程度增加,血清miR-135a-5p水平降低,TRPC1水平升高（均P<0.05）。复发组血清miR-135a-5p水平及FEV1/FVC、PEF、IL-12显著低于未复发组（均P<0.05）,TRPC1水平及IL-4、TNF-α显著高于未复发组（均P<0.05）。职业性哮喘患者血清miR-135a-5p与FEV1/FVC、PEF、IL-12呈正相关（r=0.492、0.486、0.487,均P<0.05）,与IL-4、TNF-α呈负相关（r=-0.479、-0.491,均P<0.05）;TRPC1与FEV1/FVC、PEF、IL-12呈负相关（r=-0.504、-0.494、-0.504,均P<0.05）,与IL-4、TNF-α呈正相关（r=0.481、0.495,均P<0.05）。miR-135a-5p是职业性哮喘患者1年内复发的保护因素,TRPC1是职业性哮喘患者1年内复发的独立危险因素（P<0.05）。血清miR-135a-5p、TRPC1水平单独及二者联合预测职业性哮喘患者复发的曲线下面积（area under curve,AUC）分别为0.849、0.777和0.881。结论血清miR-135a-5p、TRPC1联合检测可对职业性哮喘预后进行有效预测。

关键词: 职业性哮喘, 微小RNA-135a-5p, 瞬时受体电位通道1, 预后

Abstract: Objective To study the predictive value of serum microRNA-135a-5p（miR-135a-5p） and transient receptor potential channel 1（TRPC1） in the prognosis of occupational asthma. Methods From January 2019 to March 2021,96 occupational asthma patients admitted to Laiwu Central Hospital of Shandong Yiyang Health Group were regarded as the observation group,and were grouped into mild group,moderate group and severe group according to the severity of the disease. The patients were grouped into a recurrence group and a non-recurrence group according to whether there was recurrence within 1 year of follow-up after treatment. 92 healthy subjects served as the control group.The level of serum miR-135a-5p was detected by quantitative real-time PCR（qRT-PCR）.The enzyme-linked immunosorbent assay（ELISA） was used to detect serum levels of TRPC1 and airway inflammatory markers interleukin（IL）-4,IL-12 and tumor necrosis factor-α（TNF-α）.The forced expiratory volume in one second （FEV1） forced vital capacity（FVC）,and maximum expiratory flow（PEF） were detected by spirometry,and FEV1/FVC was calculated. The correlation of serum miR-135a-5p and TRPC1 with pulmonary function indexes and airway inflammation indexes in occupational asthma patients was analyzed by Pearson correlation.The factors affecting the recurrence of occupational asthma were analyzed by multivariate logistic regression analysis.The value of serum miR-135a-5p and TRPC1 alone and in combination in predicting recurrence in patients with occupational asthma was analyzed by receiver operating characteristic（ROC） curve. Results The level of serum miR-135a-5p in the observation group was obviously lower than that in the control group（P<0.05）,and the level of TRPC1 was obviously higher than that in the control group（P<0.05）.With the increase of disease severity,the level of serum miR-135a-5p decreased and the level of TRPC1 increased（all P<0.05）.The levels of serum miR-135a-5p,FEV1/FVC,PEF,and IL-12 in the recurrence group were obviously lower than those in the non-recurrence group（P<0.05）,and level of TRPC1,IL-4 and TNF-α were obviously higher than those in the non-recurrence group（all P<0.05）. The serum miR-135a-5p in patients with occupational asthma was positively correlated with FEV1/FVC,PEF and IL-12（r=0.492,0.486,0.487,all P<0.05）,and negatively correlated with IL-4 and TNF-α（r=-0.479,-0.491,all P<0.05）. The TRPC1 was negatively correlated with FEV1/FVC,PEF and IL-12（r=-0.504,-0.494,-0.504,all P<0.05）,and positively correlated with IL-4 and TNF-α（r=0.481,0.495,all P<0.05）.The miR-135a-5p was a protective factor for 1-year recurrence in occupational asthma patients,and TRPC1 was an independent risk factor for 1-year recurrence in occupational asthma patients（P<0.05）. The area under the curve（AUC） of serum miR-135a-5p and TRPC1 levels alone and in combination to predict recurrence in occupational asthma patients was 0.849,0.777 and 0.881,respectively. Conclusion The combined detection of serum miR-135a-5p and TRPC1 can effectively predict the prognosis of occupational asthma.

Key words: Occupational asthma, MicroRNA-135a-5p, Transient receptor potential channel 1, Prognosis

中图分类号:

R181.3⁺1

刘伟, 黄庆田, 刘娟. 血清miR-135a-5p、TRPC1水平在职业性哮喘预后评估中的价值. [J]职业与健康, 2023, 39(14): 1881-1885.

LIU Wei, HUANG Qingtian, LIU Juan. Value of serum miR-135a-5p and TRPC1 levels in prognostic assessment of occupational asthma. [J]OCCUPATION AND HEALTH, 2023, 39(14): 1881-1885.

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