莱茵衣藻辅助降血糖功能研究

职业与健康 ›› 2023, Vol. 39 ›› Issue (14): 1921-1924.

莱茵衣藻辅助降血糖功能研究

张力, 管彤, 李敏, 张大龙, 张静, 钱智勇

天津市疾病预防控制中心卫生检验检测所毒理科,天津 300011

收稿日期:2022-10-27 修回日期:2022-11-21 出版日期:2023-07-15 发布日期:2026-03-24
通信作者: 钱智勇,主任技师,E-mail：13323360260@163.com
作者简介:张力,女,副主任技师,主要从事卫生毒理学工作。

Study on auxiliary hypoglycemic function of Chlamydomonas reinhardtii

ZHANG Li, GUAN Tong, LI Min, ZHANG Dalong, ZHANG Jing, QIAN Zhiyong

Department of Toxicology,Health Inspection and Testing Institute,Tianjin Centers for Disease Control and Prevention,Tianjin,300011,China

Received:2022-10-27 Revised:2022-11-21 Online:2023-07-15 Published:2026-03-24
Contact: QIAN Zhiyong,Chief technician,E-mail：13323360260@163.com

摘要/Abstract

摘要： 目的研究莱茵衣藻对四氧嘧啶诱导胰岛素抵抗糖、脂代谢紊乱模型大鼠的辅助降血糖功效。方法 75只Wistar大鼠高热能饲料喂饲基础上,辅以腹腔注射四氧嘧啶105 mg/（kg·bw）,以构建胰岛素抵抗糖、脂代谢紊乱模型。以0.5,1.0, 2.0 g/（kg·bw·d）剂量连续33 d经口给予莱茵衣藻粉测定空腹血糖及糖耐量指标,血清胆固醇（cholesterol,CHO）、三酰甘油（triglyceride,TG）及胰岛素抵抗指数,考察莱茵衣藻对大鼠辅助降血糖功能。同时20只Wistar大鼠分为剂量组[（2.0 g/（kg·bw·d）]和对照组,连续33 d经口给予莱茵衣藻粉并喂饲普通饲料测定空腹血糖值,考察莱茵衣藻对正常大鼠的降血糖功能。结果对胰岛素抵抗糖、脂代谢紊乱模型大鼠,莱茵衣藻粉[2.0 g/（kg·bw·d）]血糖曲线下面积低于模型对照组,差异有统计学意义（P<0.05）;糖耐量指标结果阳性;各剂量组的血脂（CHO和TG）与模型对照组比较无明显升高（P>0.05）,莱茵衣藻粉对正常大鼠空腹血糖无影响（P>0.05）。结论莱茵衣藻对胰岛素抵抗糖\脂代谢紊乱大鼠具有辅助降血糖功能。

关键词: 莱茵衣藻, 降血糖, 高血糖模型, 糖耐量, 胰岛素抵抗

Abstract: Objective To study the auxiliary hypoglycemic effect of Chlamydomonas reinhardtii on alloxan-induced insulin-resistant glucose/lipid metabolism metabolism disorder model rats. Methods Totally 75 Wistar rats were fed with a high-calorie diet supplemented with intraperitoneal injection of alloxan[105 mg/（kg·bw）] to establish an insulin-resistant glucose/lipid metabolism disorder model.The Chlamydomonas reinhardtii powder was orally administered at doses of 0.5,1.0 and 2.0 g/（kg·bw·d） for 33 consecutive days,and the fasting blood glucose and glucose tolerance index,serum cholesterol（CHO）,triglyceride（TG） and insulin resistance index were measured,to investigate the auxiliary hypoglycemic effect of Chlamydomonas reinhardtii in rats.At the same time,20 Wistar rats were divided into a dose group[2.0 g/（kg·bw·d）] and a control group.They were orally given Chlamydomonas reinhardtii powder for 33 consecutive days and fed with common feed,and the fasting blood glucose was measured,to investigate the hypoglycemic effect of Chlamydomonas reinhardtii in normal rats. Results For insulin resistance glucose/lipid metabolism disorder model rats,the area under the blood glucose curve of Chlamydomonas reinhardtii powder[2.0 g/（kg·bw·d）] was lower than that of the model control group（P<0.05）,and the result of glucose tolerance index was positive.Compared with the model control group,the blood lipid CHO and TG of each dose group had no significant increase（P>0.05）. The Chlamydomonas reinhardtii powder had no effect on fasting blood glucose of normal rats. Conclusion The Chlamydomonas reinhardtii has an auxiliary hypoglycemic effect on insulin-resistant glucose/lipid metabolism disorder rats.

Key words: Chlamydomonas reinhardtii, Hypoglycemic, Hyperglycemia model, Glucose tolerance, Insulin resistance

中图分类号:

R114

张力, 管彤, 李敏, 张大龙, 张静, 钱智勇. 莱茵衣藻辅助降血糖功能研究. [J]职业与健康, 2023, 39(14): 1921-1924.

ZHANG Li, GUAN Tong, LI Min, ZHANG Dalong, ZHANG Jing, QIAN Zhiyong. Study on auxiliary hypoglycemic function of Chlamydomonas reinhardtii. [J]OCCUPATION AND HEALTH, 2023, 39(14): 1921-1924.

参考文献

[1] 杨春红,潘琳,唐茜,等.辅助降血糖功能的药食同源原料的应用概况[J].中药与临床,2022,13(1):103-107,114.
[2] International Diabetes Federation.IDF Diabetes Atlas 9th edition[EB/OL].(2019-09-01)[2019-09-01].https://diabetesatlas.org/atlas/ninth-edition/.
[3] JOANNE B C,JOSE C F.Genetics of diabetes mellitus and diabetes complications[J].Nat Rev Nephrol,2020,16(7):377-390.
[4] 方琼英,张秀玲.糖尿病现状与健康教育[J].全科护理,2012,10(5):1337-1338.
[5] 郭立新. 糖尿病的流行态势和应对策略[J].中国临床保健杂志,2020,23(4):433-436.
[6] DARWISH R,GEDI M A,AKEPACH P,et al.chlamydomonas reinhardtii is a potential food supplement with the capacity to outperform Chlorella and Spirulina[J].Appl Sci,2020,10(19):6736.
[7] MURBACH T S,GLAVITS R,ENDRES J R,et al.A toxicological evaluation of chlamydomonas reinhardtii,a green algae[J].Int J Toxicol,2018,37(1):53-62.
[8] GUEDES A C,AMARO H M,MALCATA F X.Microalgae as sources of carotenoids[J].Mar Drugs,2011,9(4):625-644.
[9] BECKER E W.Micro-algae as a source of protein[J].Biotechnol Adv,2007,25(2):207-210.
[10] 赵金瑶,陈宇坤,胡国华,等.莱茵衣藻脱腥及其在酸奶中的应用研究[J].中国食品添加剂,2021,32(9):44-50.
[11] 中华人民共和国国家食品药品监督管理局.关于印发抗氧化功能评价方法等9个保健功能评价方法的通知(国食药监保化[2012] 107号)[S/OL].(2012-04-23)[2012-05-01].http://law.foodmate.net/show-175630.html.
[12] 中华人民共和国卫生部.《保健食品检验与评价技术规范》(2003年版)[M].北京:国家食品药品监督管理局,2013:45-48.
[13] 武晗燕,毛正银,李雪梅.半乳甘露聚糖对大鼠降血糖的作用研究[J].生命科学仪器,2016(14):58-61.
[14] 黄远英,汪玉芳.燕麦β-葡聚糖辅助降血糖功能的研究[J].食品安全质量检测学报,2019,10(2):369-373.
[15] 庞增雄,黄宇锋,陈梓灵,等.葛根苦瓜类保健食品辅助降血糖功能动物实验评价[J].现代食品,2017(15):81-84.
[16] 韩彦彬,覃辉艳,梁慧莉,等.圣唐胶囊对糖尿病模型小鼠血糖影响的研究[J].中国慢性病预防与控制,2009,17(6):584-586.
[17] 生庆海,赵伟,贾艳菊,等.复合营养粉对2型糖尿病大鼠的辅助降血糖试验[J].食品与机械,2020,36(12):147-151.
[18] AOKI T T,BENBAFKA M M,DKIMURA M C,et al.Longterm intermittent intravenous insulin therapy and type 1 diabetes mellitus[J].Lancet,1993,342:515-519.
[19] 张继曼,文汉.黑豆皮花色苷的降血糖作用及其机理的研究[J].食品工业科技,2010,32(3):374-377.
[20] 冯磊,张春飞,李印彩,等.茶多糖降血糖机制研究[J].浙江预防医学,2003,15(9):10-11,13.