辽宁省生活饮用水中大肠埃希菌的检测及毒力和血清分析

摘要/Abstract

摘要： 目的了解辽宁省部分地区生活饮用水被大肠埃希菌污染的状况,并为水源性疾病的防控和生活饮用水的卫生安全监测和预警提供参考依据。方法采用多管发酵法对2010—2021年辽宁省各类生活饮用水中的大肠埃希菌微生物指标进行检测,同时采用聚合酶链反应（polymerase chain reaction,PCR）方法对分离出的大肠埃希菌进行致病性毒力基因和O抗原血清分型鉴定并分析。结果共检出大肠埃希菌78株,总样本检出率为11.84%。水样的处理方式对水样中大肠埃希菌指标检出有明显差异,未消毒样品的合格率明显低于消毒处理的水样。其中检出16株致泻大肠埃希菌,总检出率为0.024%,水源水的致泻大肠埃希菌检出率明显高于其他类样品。16株致泻大肠埃希菌中检出4株肠道致病性大肠埃希菌、5株产肠毒素大肠埃希菌和7株肠道集聚性大肠埃希菌,除5株不能分型外,其余11株分为8种血清型。毒力基因和O抗原均具有优势分型。结论辽宁省各类生活饮用水中水源水的大肠埃希菌检出率最高,生活饮用水消毒处理对降低大肠埃希菌的检出率至关重要;水源水、分散式供水、集中式供水中均检出致泻大肠埃希菌,其存在优势毒力基因,且热源稳定性基因检出比率更高,其中两种均存在优势O抗原,因此对生活饮用水中大肠埃希菌卫生指标应持续监测。

关键词: 生活饮用水, 大肠埃希菌, 致泻大肠埃希菌, 毒力基因, O抗原

Abstract: Objective To understand the situation of domestic drinking water polluted by Escherichia coli in some areas of Liaoning Province,and provide reference basis for the prevention and control of water-based diseases,as well as for the monitoring and early warning of the hygiene and safety of drinking water. Methods The microbial indicators of Escherichia coli in various kinds of drinking water in Liaoning Province from 2010 to 2021 were detected by multi tube fermentation method,and the pathogenic virulence genes and O antigen serotypes of the isolated Escherichia coli were identified and analyzed by polymerase Chain reaction（PCR） method. Results A total of 78 strains of Escherichia coli were detected,with a total sample detection rate of 11.84%. The treatment methods of water samples have significant differences in the detection of Escherichia coli indicators in water samples,and the qualification rate of non disinfected samples is significantly lower than that of disinfected water samples.Among them,16 strains of diarrhea causing Escherichia coli were detected,with a total detection rate of 0.024%. The detection rate of diarrhea causing Escherichia coli in source water was significantly higher than that in other types of samples.Four strains of Enteropathogenic Escherichia coli,five strains of Enterotoxigenic Escherichia coli and seven strains of Enteroaggregative Escherichia coli were detected from 16 strains of diarrhoeal Escherichia coli. Except five strains that could not be classified,the remaining 11 strains were classified into eight Serotype. Both virulence genes and O antigens have dominant typing. Conclusions The detection rate of Escherichia coli in various types of drinking water sources in Liaoning Province is the highest,and disinfection treatment of drinking water is crucial for reducing the detection rate of Escherichia coli.Escherichia coli causing diarrhea has been detected in source water,decentralized water supply,and centralized water supply. It has dominant virulence genes,and the detection rate of heat source stability genes is higher. Similarly,among the three kinds of Escherichia coli causing diarrhea,two of them have dominant O antigen.Therefore,the hygienic indicators of Escherichia coli in drinking water should be continuously monitored.

Key words: Drinking water, Escherichia coli, Diarrheagenic Escherichia coli, Virulence genes, O antigen

中图分类号:

R123

魏彤竹, 李雪, 张旭, 于淼, 王伟杰, 孙婷婷. 辽宁省生活饮用水中大肠埃希菌的检测及毒力和血清分析. [J]职业与健康, 2023, 39(16): 2193-2197.

WEI Tongzhu, LI Xue, ZHANG Xu, YU Miao, WANG Weijie, SUN Tingting. Detection,virulence and serum analysis of Escherichia coli in drinking water in Liaoning Province. [J]OCCUPATION AND HEALTH, 2023, 39(16): 2193-2197.

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