关键词: 枸杞多糖, 肝纤维化, 肠道菌群, 炎性因子, 纤维化相关因子

Abstract: Objective To analyze the effects of lycium barbarum polysaccharides（LBP） on the contents of designated flora in intestinal tract,inflammatory factors in blood and fibrosis related factors in liver tissue of liver fibrosis mice,explore the protective mechanism of LBP on liver fibrosis. Methods 20 mouse models of hepatic fibrosis were established by ligating their common bile duct,and they were equally divided into model group and LBP treatment group. Another 10 healthy mice were selected as the control group. The mice in LBP treatment group were given intragastric administration 5 mL solution with 30 μg/g LBP every day from the third week after operation. The mice in control group and model group were given 5 mL of tridistilled water by gavage at the same time every day. Three groups were treated for two weeks. The qRT-PCR was used to detect the changes of designated flora in intestinal tract. The enzyme-linked immunosorbent assay（ELISA） was used to detect the contnts of inflammatory factors in blood,including interleukin-1β（IL-1β）,interleukin-6（IL-6）,interleukin-10（IL-10） and tumor necrosis factor-α（TNF-α）. The Western blot analysis was used to analyze the relative value of fibrosis related factors in liver tissue,including type I collagen,α-smooth muscle actin（α-SMA） and transforming growth factor-β1（TGF-β1）. Results Compare with the control group,different sizes and quantities necrotic areas appeared in the liver of the model group,and the content of connective tissue and the number of bile ducts increased, Escherichia coli（179.27±30.93） and Clostridium perfringens（234.93±40.17） in intestinal contents increased,Bifidobacterium（15.86±4.98） and Lactobacillus（28.12±7.30） decreased（all P<0.01）,the contents of blood inflammatory factor IL-1β[（46.30±4.15）pg/mL],IL-6[（19.18±2.04）pg/mL] and TNF-α[（85.28±7.49）ng/mL] increased,while content of IL-10[（28.68±2.58）ng/mL] decreased（all P<0.01）,the relative value of fibrosis related factors type I collagen（1.209±0.053）,α-SMA（0.416±0.012） and TGF-β1（0.675±0.018） increased（all P<0.05）. Compare with the model group,the area and quantity of intrahepatic necrosis in LBP treatment group decreased,the content of connective tissue and the number of bile ducts decreased,Escherichia coli（121.56±20.25） and Clostridium perfringens（117.83±24.50） in intestinal contents decreased,Bifidobacterium（60.08±13.60） and Lactobacillus（92.50±19.42） increased（all P<0.01）,the contents of blood inflammatory factor IL-1β[（19.33±1.68）pg/mL],IL-6[（11.79±1.70）pg/mL] and TNF-α[（50.24±4.90）ng/mL] decreased,while content of IL-10[（58.61±5.17）ng/mL] increased（all P<0.01）,the relative value of fibrosis related factors type I collagen（0.760±0.020）,α-SMA（0.176±0.008） and TGF-β1（0.420±0.016） decreased（all P<0.05）. Conclusion The lycium barbarum polysaccharides can reduce the degree of liver fibrosis by regulating the designated flora in the intestinal tract and the content of inflammatory factors in the blood of mice.

Key words: Lycium barbarum polysaccharides, Hepatic fibrosis, Intestinal flora, Inflammatory factors, Fibrosis related factors