除草剂莠去津暴露对人类和其他哺乳动物健康影响的研究进展

职业与健康 ›› 2024, Vol. 40 ›› Issue (4): 547-553.

除草剂莠去津暴露对人类和其他哺乳动物健康影响的研究进展

白亚男^1,2, 周蓉¹, 邢巍巍³, 虞悦¹, 戴传超², 卜元卿^1,4

1.生态环境部南京环境科学研究所,国家环境保护农药环境评价与污染控制重点实验室,江苏南京 210042;
2.南京师范大学生命科学学院,江苏南京 210023;
3.大连市生态环境事务服务中心,辽宁大连 116000;
4.南京信息工程大学江苏省大气环境与装备技术协同创新中心,江苏南京 210044

收稿日期:2023-05-19 修回日期:2023-07-24 出版日期:2024-02-15 发布日期:2026-03-12
通信作者: 卜元卿,研究员,E-mail：byq@nies.org
作者简介:白亚男,女,在读硕士研究生,研究方向为环境毒理学与微生物生态。
基金资助:
国家重点研发计划（2018YFC1801105）;生态环境部事业费项目（农村与农业环境保护监督管理）

Research progress on healthy effects of herbicide atrazine exposure on human and other mammal

BAI Yanan^1,2, ZHOU Rong¹, XING Weiwei³, YU Yue¹, DAI Chuanchao², BU Yuanqing^1,4

1. Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Ministry of Ecology and Environment, Nanjing Jiangsu, 210042, China;
2. Colloge of Life Sciences, Nanjing Normal University, Nanjing Jiangsu, 210023, China;
3. Dalian Eco-environmental Affairs Service Center, Dalian Liaoning, 116000, China;
4. Jiangsu Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, Nanjing University of Information Science & Technology, Nanjing Jiangsu, 210044, China

Received:2023-05-19 Revised:2023-07-24 Online:2024-02-15 Published:2026-03-12
Contact: BU Yuanqing,Researcher,E-mail：byq@nies.org

摘要/Abstract

摘要： 目的莠去津（Atrazine,ATZ）是一种在世界范围内广泛使用的三嗪类除草剂,在有ATZ使用历史的地区水体中均检测到ATZ的残留。如果长期持续超量使用,环境中ATZ及其主要降解物质会通过食物链转移,对人类及其他哺乳动物的生殖发育、内分泌、神经、免疫与遗传系统有潜在的毒性作用,进而诱发癌症。因此,本文主要综述了ATZ暴露对人类及其他哺乳动物生殖发育、内分泌、神经、免疫、遗传系统以及潜在致癌性影响的研究进展,并展望了ATZ使用的环境健康风险,提出了ATZ的环境管理建议,对于进一步加强ATZ的使用具有重要意义。

关键词: 莠去津, 环境健康, 生殖发育毒性, 内分泌干扰, 神经毒性, 遗传毒性, 免疫毒性, 致癌性

Abstract: Objective Atrazine（ATZ） is a triazine herbicide widely used in the world.Residues of ATZ have been detected in water bodies in regions with a history of ATZ use.If it is used in excess for a long time,ATZ and its main degradation substances in the environment will be transferred through the food chain,which has potential toxic effects on the reproductive development,endocrine,nervous,immune and genetic systems of humans and other mammals,and then induce cancer.Therefore,this paper mainly reviews the research progress of ATZ exposure on human and other mammalian reproductive development,endocrine,nervous,immune,genetic system and potential carcinogenicity,and prospects the environmental health risks of ATZ use,and puts forward environmental management suggestions of ATZ,which is of great significance to further strengthen the use of ATZ.

Key words: Atrazine, Environmental health, Reproductive and developmental toxicity, Endocrine disruption, Neurotoxicity, Genotoxicity, Immunotoxicity, Carcinogenicity

中图分类号:

R114

白亚男, 周蓉, 邢巍巍, 虞悦, 戴传超, 卜元卿. 除草剂莠去津暴露对人类和其他哺乳动物健康影响的研究进展. [J]职业与健康, 2024, 40(4): 547-553.

BAI Yanan, ZHOU Rong, XING Weiwei, YU Yue, DAI Chuanchao, BU Yuanqing. Research progress on healthy effects of herbicide atrazine exposure on human and other mammal. [J]OCCUPATION AND HEALTH, 2024, 40(4): 547-553.

参考文献

[1] 陈学国,滕姣,赵丹,等. 莠去津及代谢物研究进展[J].福建分析测试,2019,28(2):23-28.
[2] DE ALBUQUERQUE F P,DE OLIVEIRA J L,MOSCHINI-CARLOS V,et al.An overview of the potential impacts of atrazine in aquatic environments:Perspectives for tailored solutions based on nanotechnology[J].Sci Total Environ,2020,700:134868.
[3] WANG A,HU X,WAN Y,et al.A nationwide study of the occurrence and distribution of atrazine and its degradates in tap water and groundwater in China:Assessment of human exposure potential[J].Chemosphere,2020,252:126533.
[4] 张剑峰,刘静晶,王晓婧,等.黑龙江省玉米产区浅层地下水中莠去津及其代谢产物调查[J].中国公共卫生管理,2020,36(1):36-39.
[5] DOU R,SUN J,DENG F,et al.Contamination of pyrethroids and atrazine in greenhouse and open-field agricultural soils in China[J].Sci Total Environ,2020,701:134916.
[6] OLATOYE I O,OKOCHA R C,ORIDUPA O A,et al.Atrazine in fish feed and african catfish(Clarias gariepinus)from aquaculture farms in Southwestern Nigeria[J].Heliyon,2021,7(2):e06076.
[7] 滕姣. 三嗪类除草剂预处理及检验方法研究进展[J].福建分析测试,2021,30(6):18-22.
[8] GORE A C,CHAPPELL V A,FENTON S E,et al.EDC-2:The Endocrine Society's Second Scientific Statement on Endocrine-Disrupting Chemicals[J].Endocr Rev,2015,36(06):E1-E150.
[9] FORADORI C D,ZIMMERMAN A D,HINDS L R,et al.Atrazine inhibits pulsatile gonadotropin-releasing hormone(GnRH) release without altering GnRH messenger RNA or protein levels in the female rat[J].Biol Reprod,2013,88(1):9.
[10] GOLDMAN J M,DAVIS L K,MURR A S,et al.Atrazine-induced elevation or attenuation of the LH surge in the ovariectomized,estrogen-primed female rat:Role of adrenal progesterone[J].Reproduction,2013,146(4):305-314.
[11] KIMURA M,ISHII M N,SEKI N,et al.Reduction of Kiss1 expression in the anteroventral periventricular nucleus is associated with atrazine-induced attenuation of the luteinizing hormone surge in female rats[J].Biol Reprod,2019,100(1):41-48.
[12] TRENTACOSTE S V,FRIEDMANN A S,YOUKER R T,et al.Atrazine effects on testosterone levels and androgen-dependent reproductive organs in peripubertal male rats[J].J Androl,2001,22(1):142-148.
[13] SANDERSON J T,BOERMA J,LANSBERGEN G W,et al.Induction and inhibition of aromatase(CYP19) activity by various classes of pesticides in H295R human adrenocortical carcinoma cells[J].Toxicol Appl Pharmacol,2002,182(1):44-54.
[14] HOLLOWAY A C,ANGER D A,CRANKSHAW D J,et al.Atrazine-induced changes in aromatase activity in estrogen sensitive target tissues[J].J Appl Toxicol,2008,28(3):260-270.
[15] TINFO N S,HOTCHKISS M G,BUCKALEW A R,et al.Understanding the effects of atrazine on steroidogenesis in rat granulosa and H295R adrenal cortical carcinoma cells[J].Reprod Toxicol,2011,31(2):184-193.
[16] POGRMIC K,FA S,DAKIC V,et al.Atrazine oral exposure of peripubertal male rats downregulates steroidogenesis gene expression in Leydig cells[J].Toxicol Sci,2009,111(1):189-197.
[17] POGRMIC-MAJKIC K,FA S,DAKIC V,et al.Upregulation of peripubertal rat Leydig cell steroidogenesis following 24 h in vitro and in vivo exposure to atrazine[J].Toxicol Sci,2010,118(1):52-60.
[18] RIFFLE B W,KLINEFELTER G R,COOPER R L,et al.Novel molecular events associated with altered steroidogenesis induced by exposure to atrazine in the intact and castrate male rat[J].Reprod Toxicol,2014,47:59-69.
[19] ABDEL AZIZ R L,ABDEL-WAHAB A,ABO EL-ELA F I,et al.Dose-dependent ameliorative effects of quercetin and l-Carnitine against atrazine- induced reproductive toxicity in adult male Albino rats[J].Biomed Pharmacother,2018,102:855-864.
[20] KALE O E,OYESOLA T O,RAJI F S.Celecoxib,a cyclooxygenase-2 inhibitor,offers chemoprevention against reproductive and neurobehavioural abnormalities induced by atrazine in male Wistar rats[J].Environ Toxicol Pharmacol,2018,58:84-97.
[21] WALTERS J L,LANSDELL T A,LOOKINGLAND K J,et al.The effects of gestational and chronic atrazine exposure on motor behaviors and striatal dopamine in male Sprague-Dawley rats[J].Toxicol Appl Pharmacol,2015,289(2):185-192.
[22] BELLONI V,DESSÌ-FULGHERI F,ZACCARONI M,et al.Early exposure to low doses of atrazine affects behavior in juvenile and adult CD1 mice[J].Toxicology,2011,279(1-3):19-26.
[23] LI Y,SUN Y,YANG J,et al.The long-term effects of the herbicide atrazine on the dopaminergic system following exposure during pubertal development[J].Mutat Res Genet Toxicol Environ Mutagen,2014,763:23-29.
[24] 李家男,宋肖垚,吴沿萍,等.阿特拉津对大鼠多巴胺神经元凋亡相关因子表达的影响[J].癌变·畸变·突变,2015,27(2):121-124.
[25] SUN Y,LI Y S,LI B,et al.A study of the age-related effects of lactational atrazine exposure[J].Reprod Toxicol,2017,69:230-241.
[26] COBAN A,FILIPOV N M.Dopaminergic toxicity associated with oral exposure to the herbicide atrazine in juvenile male C57BL/6 mice[J].J Neurochem,2007,100(5):1177-1187.
[27] GIUSI G,FACCIOLO R M,CANONACO M,et al.The endocrine disruptor atrazine accounts for a dimorphic somatostatinergic neuronal expression pattern in mice[J].Toxicol Sci,2006,89(1):257-264.
[28] ALLEN J P,HATHWAY G J,CLARKE N J,et al.Somatostatin receptor 2 knockout/lacZ knockin mice show impaired motor coordination and reveal sites of somatostatin action within the striatum[J].Eur J Neurosci,2003,17(9):1881-1895.
[29] ZHAO S,LIU J,ZHAO F,et al.Sub-acute exposure to the herbicide atrazine suppresses cell immune functions in adolescent mice[J].Biosci Trends,2013,7(4):193-201.
[30] LEE G H,CHOI K C.Adverse effects of pesticides on the functions of immune system[J].Comp Biochem Physiol C Toxicol Pharmacol,2020, 235:108789.
[31] LEE E J,JANG Y,KANG K,et al.Atrazine induces endoplasmic reticulum stress-mediated apoptosis of T lymphocytes via the caspase-8-dependent pathway[J].Environ Toxicol,2016,31(8):998-1008.
[32] THUESON L E,EMMONS T R,BROWNING D L,et al.In vitro exposure to the herbicide atrazine inhibits T cell activation,proliferation,and cytokine production and significantly increases the frequency of Foxp3+ regulatory T cells[J].Toxicol Sci,2015,143(2):418-429.
[33] 赵菁,刘剑,张敏,等.除草剂阿特拉津的免疫毒性及其对大鼠免疫功能的影响[J].吉林大学学报(医学版),2018,44(6):1163-1168.
[34] JESTADI D B,PHANIENDRA A,BABJI U,et al.Effects of short term exposure of atrazine on the liver and kidney of normal and diabetic rats[J].J Toxicol,2014,2014:536759.
[35] TIMMINS J M,OZCAN L,SEIMON T A,et al.Calcium/calmodulin-dependent protein kinase II links ER stress with Fas and mitochondrial apoptosis pathways[J].J Clin Invest,2009,119(10):2925-2941.
[36] GAO S,WANG Z,ZHANG C,et al.Oral Exposure to Atrazine Induces Oxidative Stress and Calcium Homeostasis Disruption in Spleen of Mice[J].Oxid Med Cell Longev,2016,2016:7978219.
[37] PRAKASH S.Artificial Cells,Cell Engineering and Therapy[M].Elsevier Science:Woodhead Publishing,2007:1.
[38] KARROW N A,MCCAY J A,BROWN R D,et al.Oral exposure to atrazine modulates cell-mediated immune function and decreases host resistance to the B16F10 tumor model in female B6C3F1 mice[J].Toxicology,2005,209(1):15-28.
[39] 马思佳,陈锦瑶,张立实.阿特拉津对小鼠腹腔巨噬细胞功能影响的体外研究[J].卫生研究,2015,44(6):949-953.
[40] CRAGIN L A,KESNER J S,BACHAND A M,et al.Menstrual cycle characteristics and reproductive hormone levels in women exposed to atrazine in drinking water[J].Environ Res,2011,111(8):1293-1301.
[41] YUN Y,LEE S,SO C,et al.Oocyte Development and Quality in Young and Old Mice following Exposure to Atrazine[J].Environ Health Perspect,2022,130(11):117007.
[42] 聂果,吴春先,高立明,等.莠去津的生态毒理学及其环境行为学研究进展[J].现代农药,2007(4):32-37,54.
[43] KETTLES M K,BROWNING S R,PRINCE T S,et al.Triazine herbicide exposure and breast cancer incidence:an ecologic study of Kentucky counties[J].Environ Health Perspect,1997,105(11):1222-1227.
[44] LHEUREUX S,GOURLEY C,VERGOTE I,et al.Epithelial ovarian cancer[J].Lancet,2019,393(10177):1240-1253.
[45] ALBANITO L,MADEO A,LAPPANO R,et al.G protein-coupled receptor 30(GPR30) mediates gene expression changes and growth response to 17beta-estradiol and selective GPR30 ligand G-1 in ovarian cancer cells[J].Cancer Res,2007,67(4):1859-1866.
[46] 刘剑. 除草剂阿特拉津对人卵巢癌Skov3细胞增殖转移能力影响的体外研究[D].吉林:吉林大学,2013.
[47] 孟顺龙. 除草剂阿特拉津对鲫鱼的毒性影响研究[D].南京:南京农业大学,2007.
[48] ROLOFF B D,BELLUCK D A,MEISNER L F.Cytogenetic studies of herbicide interactions in vitro and in vivo using atrazine and linuron[J].Arch Environ Contam Toxicol,1992,22(3):267-271.
[49] RIBAS G,SURRALLÉS J,CARBONELL E,et al.Lack of genotoxicity of the herbicide atrazine in cultured human lymphocytes[J].Mutat Res,1998,416(1-2):93-99.
[50] 张梦云,郭爱萍,王显菊,等.莠去津对雌性幼年大鼠生殖系统发育的毒性研究[J].环境与健康杂志,2013,30(6):527-529,565.
[51] RAYNER J L,ENOCH R R,FENTON S E.Adverse effects of prenatal exposure to atrazine during a critical period of mammary gland growth[J].Toxicol Sci,2005,87(1):255-266.
[52] RAYNER J L,WOOD C,FENTON S E.Exposure parameters necessary for delayed puberty and mammary gland development in Long-Evans rats exposed in utero to atrazine[J].Toxicol Appl Pharmacol,2004,195(1):23-34.
[53] NAROTSKY M G,BEST D S,GUIDICI D L,et al.Strain comparisons of atrazine-induced pregnancy loss in the rat[J].Reprod Toxicol,2001, 15(1):61-69.
[54] ROSENBERG B G,CHEN H,FOLMER J,et al.Gestational exposure to atrazine:Effects on the postnatal development of male offspring[J].J Androl,2008,29(3):304-311.
[55] SONG Y,JIA Z C,CHEN J Y,et al.Toxic effects of atrazine on reproductive system of male rats[J].Biomed Environ Sci,2014,27(4):281-288.