生活饮用水中氧苯酮的SPE-HPLC-MS检测方法

职业与健康 ›› 2025, Vol. 41 ›› Issue (10): 1351-1355.

生活饮用水中氧苯酮的SPE-HPLC-MS检测方法

张文娇¹, 何远东², 李鸣¹, 姚于勤¹, 史莹¹

1.四川大学华西公共卫生学院/华西第四医院,四川成都 610041;
2.成都市双流区第一人民医院医务科,四川成都 610299

收稿日期:2024-05-23 修回日期:2024-12-12 出版日期:2025-05-15 发布日期:2025-12-18
通信作者: 史莹,讲师,E-mail：jennysing2013@163.com
作者简介:张文娇,女,在读硕士研究生,研究方向为卫生理化检验。

Determination of oxybenzone in drinking water by SPE-HPLC-MS

ZHANG Wenjiao¹, HE Yuandong², LI Ming¹, YAO Yuqin¹, SHI Ying¹

1. West China School of Public Health,Sichuan University/West China Fourth Hospital,Chengdu,Sichuan 610041,China;
2. Medical Department,Chengdu Shuangliu Districtthe First People's Hospital,Chengdu,Sichuan 610299,China

Received:2024-05-23 Revised:2024-12-12 Online:2025-05-15 Published:2025-12-18
Contact: SHI Ying,Lecturer,E-mail：jennysing2013@163.com

摘要/Abstract

摘要： 目的建立生活饮用水中氧苯酮残留检测的固相萃取-高效液相色谱-串联质谱法,并对实际生活饮用水中氧苯酮的残留进行检测。方法采用固相萃取进行样品前处理,选用C₁₈色谱柱、甲醇和超纯水作为流动相,二元高压梯度洗脱,多反应监测模式监测,电喷雾正离子模式测定,建立高效液相色谱-串联质谱法。结果氧苯酮的线性范围为0.200~500 μg/L,回归方程为y=26 578x-4 882,r=0.998 8,相对标准偏差为3.44%,方法检出限为0.005 ng/L,定量限为0.017 ng/L,加标平均回收率为79.9%~101.0%。对成都市市内所采水样进行氧苯酮残留检测,均检测出残留,其平均浓度为6.43 ng/L。结论建立的检测方法灵敏、快速,能够满足生活饮用水中氧苯酮残留量的检测工作要求,可为后续氧苯酮残留检测研究提供实验基础。

关键词: 氧苯酮, 生活饮用水, 固相萃取, 高效液相色谱-串联质谱法

Abstract: Objective To establish a solid phase extraction-high performance liquid chromatography-tandem mass spectrometry （SPE-HPLC-MS/MS） method for the determination of oxybenzone residues in domestic drinking water,and to detect the residual oxybenzoneinactual domestic drinking water. Methods HPLC-MS/MS method was established by using SPE for sample pretreatment,a C₁₈ column,methanol and ultrapure water as the mobile phases,a binary high-pressure gradient elution,multi-reaction monitoringmode for monitoring,and electrospraying+mode for determining. Results The linear range of oxybenzone was 0.200-500 μg/L with the regression equation of y=26 578x-4882,r=0.998 8,the relative standard deviation was 3.44%,and the limits of detection of the method were 0.005 0 ng/L,the limits of quantification were 0.017 ng/L,and the recoveries of the spiked standards ranged from 79.9 to 101.0%. Oxybenzone residues were detected in water samples taken in Chengdu,with average concentration of 6.43 ng/L. Conclusion The established method is sensitive,rapid,and can meet the requirements for the detection of oxybenzone residue in drinking water,and can provide an experimental basis for the subsequent study of oxybenzone residue detection.

Key words: Oxybenzone, Drinking water, Solid phase extraction, High performance liquid chromatography-tandem mass spectrometry

中图分类号:

R115

张文娇, 何远东, 李鸣, 姚于勤, 史莹. 生活饮用水中氧苯酮的SPE-HPLC-MS检测方法. [J]职业与健康, 2025, 41(10): 1351-1355.

ZHANG Wenjiao, HE Yuandong, LI Ming, YAO Yuqin, SHI Ying. Determination of oxybenzone in drinking water by SPE-HPLC-MS. [J]OCCUPATION AND HEALTH, 2025, 41(10): 1351-1355.

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