连续流动注射法快速检测尿液中氰化物

职业与健康 ›› 2025, Vol. 41 ›› Issue (16): 2195-2198.

连续流动注射法快速检测尿液中氰化物

翁月阳, 李昌安, 张克才

合肥市疾病预防控制中心理检科,安徽合肥 230091

收稿日期:2025-01-02 修回日期:2025-01-22 出版日期:2025-08-15 发布日期:2025-12-13
通信作者: 李昌安,副主任检验技师,E-mail：1164797569@qq.com
作者简介:翁月阳,女,主管检验技师,主要从事水质和食品安全风险监测相关工作。

Rapid detection of cyanide in urine using continuous flow injection method

WENG Yueyang, LI Changan, ZHANG Kecai

Physical Inspection Department,Hefei Municipal Center for Disease Control and Prevention,Auhui,Hefei 230091,China

Received:2025-01-02 Revised:2025-01-22 Online:2025-08-15 Published:2025-12-13
Contact: LI Changan,Deputy chief inspection technician,E-mail：1164797569@qq.com

摘要/Abstract

摘要： 目的通过氢氧化钠溶液稀释碱解建立连续流动注射法高效快速的检测尿液中氰化物的方法,并分析稀释倍数、氢氧化钠溶液浓度和碱解方式对尿液中氰化物测定的影响。方法尿液样品在冷冻离心后用适当浓度的氢氧化钠溶液稀释适当的倍数后通过连续流动注射分析仪测定尿样中的氰化物含量。结果该方法检测氰化物在0~0.200 mg/L浓度范围内呈现良好的线性关系,相关系数为0.999 6,检出限为0.005 4 mg/L。对尿液样本进行低、中、高3个不同浓度的加标回收实验,结果显示加标回收率为84.0%~92.0%,相对标准偏差为2.341%~6.206%。结论连续流动注射法检测尿液中的氰化物灵敏度高、稳定性好,无需复杂的前处理和衍生过程,全程在线蒸馏安全性好,可以用于尿液中氰化物的日常检测。

关键词: 连续流动, 氰化物, 尿液, 直接稀释

Abstract: Objective To establish a rapid and efficient method for the detection of cyanide in urine with continuous flow inject analysis by diluting alkaline hydrolysis with sodium hydroxide solution,analyze the effects of dilution ratio,sodium hydroxide solution concentration,and alkaline hydrolysis method on the determination of cyanide in urine. Methods After refrigerated centrifugation,the urine samples were diluted with a suitable concentration of sodium hydroxide solution to an appropriate multiple,and a continuous flow injection analyzer was use to determine the cyanide content in the urine sample. Results This research showed a good line relationship for detecting cyanide in the concentration range of 0-0.200 mg/L,with a linear correlation coefficient of 0.999 6. The detection limit of the method was 0.005 4 mg/L. The spiked recovery experiment on urine samples at three different concentrations（low,medium,and high） were conducted,and the results showed that the recoveries ranged from 84.0% to 92.0%. The relative standard deviations of this method were 2.341% to 6.206%. Conclusion The continuous flow injection method for the detection of cyanide in urine has high sensitivity and good stability without complex pretreatment and derivation processes,and the whole process of online distillation is safe,which can be used for routine detection of cyanide in urine.

Key words: Continuous flow, Cyanide, Urine, Direct dilution

中图分类号:

R115

翁月阳, 李昌安, 张克才. 连续流动注射法快速检测尿液中氰化物. [J]职业与健康, 2025, 41(16): 2195-2198.

WENG Yueyang, LI Changan, ZHANG Kecai. Rapid detection of cyanide in urine using continuous flow injection method. [J]OCCUPATION AND HEALTH, 2025, 41(16): 2195-2198.

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