Objective To investigate the status of work connectedness behavior,work-family conflict and job burnout among obstetrics and gynecology nurses,analyze the influence of connectedness behavior and work-family conflict on occupational burnout. Methods From January to October 2024,178 obstetrics and gynecology nurses from 12 grade-A tertiary general hospitals in Hubei Province were randomly selected by the random sampling method as the research subjects. The work connectedness behavior scale,work-family conflict scale and job burnout scale were used to investigate and analyze the work connectedness behavior,work-family conflict and job burnout. Results The average score of work connectedness behavior,work-family conflict and job burnout among 178 obstetrics and gynecology nurses was (40.34±6.92),(60.94±10.32) and (60.47±12.36)points,respectively. The influencing factors of work connectedness behavior,work-family conflict and job burnout among obstetrics and gynecology nurses were age,professional title,years of work,average monthly income,average number of monthly shifts and marital status(all P<0.05). The work connectedness behavior and work-family conflict of obstetrics and gynecology nurses were positively correlated with job burnout(r=0.586,0.614,both P<0.01),and the work connectedness behavior was positively correlated with work-family conflict(r=0.569,P<0.01). The work-family conflict played a partial mediating effect between the work connectedness behavior and job burnout of obstetrics and gynecology nurses,and the mediating effect strength value was 0.281. Conclusion The work-family conflict has a partial mediating effect between the work connectedness behavior and job burnout of obstetrics and gynecology nurses,and reducing the level of work connectedness behavior and work-family conflict can help reduce their job burnout level.